'Side Population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells
'Side Population' cells in adult mouse testis express Bcrp1 gene and are enriched in spermatogonia and germinal stem cells
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DOI：
10.1242/dev.00918
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发表时间：
2004-01-01
期刊：
影响因子：
4.6
通讯作者：
Allemand, I
中科院分区：
文献类型：
--
作者：
Lassalle, B;Bastos, H;Allemand, I
各种体细胞组织（骨髓、骨骼肌）中的干细胞可以通过基于Hoechst 33342流出的“侧群”标记物来鉴定。我们发现，小鼠睾丸细胞也显示出表达Bcrp 1 mRNA的“侧群”，Bcrp 1 mRNA是造血细胞中负责Hoechst流出的ABC转运蛋白。通过特异性BCRP 1抑制剂Ko 143对Hoechst外排的抑制表明，germinal“侧群”表型依赖于BCRP 1活性。两个定义明确的模型改变精子发生（W/W-V突变体和隐睾雄性小鼠）和RNA表达的分化标志物的研究分析表明，germinal '侧人口'包含精原细胞。此外，α（6）-整联蛋白和Stra 8干细胞标志物在“侧群”中表达。体内再增殖。该试验清楚地证明了成年小鼠睾丸的“侧群”高度富集雄性生殖干细胞。
Stem cells in various somatic tissues (bone marrow, skeletal muscle) can be identified by the 'Side Population' marker based on Hoechst 33342 efflux. We show that mouse testicular cells also display a 'Side Population' that express Bcrp1 mRNA, the ABC transporter responsible for Hoechst efflux in hematopoietic cells. Inhibition of Hoechst efflux by specific BCRP1 inhibitor Ko143 show that germinal 'Side Population' phenotype is dependent on BCRP1 activity. Analysis of two well-defined models of altered spermatogenesis (W/W-v mutants and cryptorchid male mice) and RNA expression studies of differentiation markers demonstrate that germinal 'Side Population' contains spermatogonial cells. In addition, alpha(6)-integrin and Stra8 germinal stem cell markers, are expressed in the 'Side Population'. In vivo repopulation. assay clearly establishes that testis 'Side Population' in adult mice is highly enriched in male germ stem cells.