Regulation of collagen production in fibroblasts cultured from normal and phenytoin-induced hyperplastic human gingiva.
Regulation of collagen production in fibroblasts cultured from normal and phenytoin-induced hyperplastic human gingiva.
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正常和苯妥英诱导的增生性人牙龈培养的成纤维细胞中胶原蛋白产生的调节。
DOI：
10.1111/j.1600-0765.1988.tb01343.x
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发表时间：
1988
影响因子：
3.5
通讯作者：
Page,RC
中科院分区：
文献类型：
--
作者：
Narayanan,AS;Meyers,DF;Page,RC
我们研究了从正常和苯妥英诱导的增生性人牙龈中获得的成纤维细胞中胶原蛋白的产生是如何调节的。胶原蛋白的产生被确定为胶原酶可消化的放射性，并通过向培养物中加入标记的前胶原蛋白和在溶酶体抑制剂存在下进行脉冲标记来检查降解。使用[35 S]-UTP标记的proα[1]探针测量胶原mRNA水平。正常和苯妥英诱导的成纤维细胞没有降解胶原蛋白胞外和溶酶体抑制剂没有提高胶原蛋白的生产在任何文化。培养物的胶原蛋白产生与mRNA水平相关，在3个苯妥英诱导的成纤维细胞中，有2个细胞产生的胶原蛋白比其他细胞多。胶原mRNA水平较高。我们得出结论，牙龈成纤维细胞中的胶原蛋白产生主要受mRNA水平的调节，苯妥英诱导的增生牙龈细胞的胶原蛋白过度产生是由于胶原蛋白mRNA稳态水平的增加而不是胶原蛋白降解的减少。
We have studied how collagen production is regulated in fibroblasts obtained from normal and phenytoin‐induced hyperplastic human gingiva. Collagen production was determined as collagenase digestible radioactivity and degradation was examined by adding labelled procollagen to the cultures and by pulselabelling in the presence of lysosomal inhibitors. Collagen mRNA levels were measured using a [35S]‐UTP labelled proα[1] probe. The normal and phenytoininduced fibroblasts did not degrade collagen extracellularly and lysosomal inhibitors did not enhance collagen production in either culture. Collagen production by the cultures correlated with mRNA levels, and in 2 of 3 phenytoin‐induced fibroblasts, which produced more collagen than other cells. collagen mRNA levels were higher. We conclude that collagen production in gingival fibroblasts is primarily regulated by the mRNA levels and that overproduction of collagen by cells from phenytoin‐induced hyperplastic gingiva results from an increased steady state level of collagen mRNA and not decreased collagen degradation.