CDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera; Rhinotermitidae).
CDNA cloning of aquaporin gene expressed in the digestive tract of the Formosan subterranean termite, Coptotermes formosanus Shiraki (Isoptera; Rhinotermitidae).
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DOI：
10.1303/aez.2009.315
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发表时间：
2009-05
影响因子：
1.3
通讯作者：
K. Kambara;Y. Takematsu;M. Azuma;J. Kobayashi
中科院分区：
文献类型：
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作者：
K. Kambara;Y. Takematsu;M. Azuma;J. Kobayashi
从台湾乳白蚁消化道中获得了属于主要内在蛋白（MIP）家族的水通道蛋白（AQP）的cDNA。该cDNA编码一个AQP同源物，命名为CfAQP，具有249个氨基酸。RT-PCR分析表明，CfAQP在白蚁消化道和其他组织中均有表达，提示CfAQP不仅在白蚁体内水循环过程中起重要作用，而且在其他重要的水分运输过程中也起重要作用。利用17种昆虫的71个MIP序列对CfAQP进行系统发育分析，结果表明，昆虫MIP可分为4个类群，命名为昆虫MIP类群1 ~类群4，CfAQP是脊椎动物AQP的姐妹类群类群1的成员。
A cDNA of aquaporin (AQP, water channel) belonging to the major intrinsic protein (MIP) family was obtained from the digestive tract of the Formosan subterranean termite, Coptotermes formosanus. The cDNA encoded an AQP homolog designated as CfAQP with 249 amino acids. RT-PCR analysis revealed the expression of CfAQP in the digestive tract and other tissues, suggesting that CfAQP may play important roles not only in water recycling but also other essential water transport processes in the termite. Phylogenetic analysis of CfAQP with 71 insect MIP sequences from 17 species indicated that insect MIPs could be classified into 4 groups, designated as insect MIP Group 1 to Group 4, and that CfAQP is a member of Group 1, a sister group of vertebrate AQPs.