Data of whole genome sequencing of five garden asparagus (Asparagus officinalis) individuals with the MinION nanopore sequencer
Data of whole genome sequencing of five garden asparagus (Asparagus officinalis) individuals with the MinION nanopore sequencer
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DOI：
10.1016/j.dib.2019.104838
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发表时间：
2019-11
期刊：
影响因子：
1.2
通讯作者：
D. Tsugama;K. Fujino
中科院分区：
文献类型：
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作者：
D. Tsugama;K. Fujino
芦笋（Asparagus officinalis）是一种多年生雌雄异株作物。基因组DNA样品从五个A.将不同性别和表型的个体进行药用化，并用MinION纳米孔测序仪进行测序。获得的数据为1.5- 5Gb/样品，并且所有样品的平均读取长度大于1.4kb。将所得读数映射到现有A.药用植物基因组序列。现有的A。将officinalistranscript序列映射到MinION衍生的读数。在这些定位结果的基础上，通过基因组PCR，然后进行桑格测序，确定了先前未定位到现有基因组任何区域的5个部分基因片段的侧翼序列。这些序列能够估计这五个部分基因片段的基因组位置。MinION衍生的数据和五个基因片段的侧翼序列分别保存在NCBI（国家生物技术信息中心）SRA（序列读取档案）数据库和NCBI核苷酸数据库中。
Garden asparagus (Asparagus officinalis) is a perennial, dioecious crop. Genomic DNA samples were prepared from fiveA. officinalisindividuals that differ in sex and phenotypes, and sequenced with the MinION nanopore sequencer. The obtained data were 1.5–5 Gb/sample, and the average read length was larger than 1.4 kb for all the samples. The resulting reads were mapped to the existingA. officinalisgenome sequence. The existingA. officinalistranscript sequences were mapped to the MinION-derived reads. On the basis of these mapping results, flanking sequences of five partial gene fragments that previously had not been mapped to any region of the existing genome were determined by genomic PCR followed by Sanger sequencing. These sequences enabled to estimate the genomic positions of those five partial gene fragments. The MinION-derived data and the flanking sequences of the five gene fragments were deposited in the NCBI (National Center for Biotechnology Information) SRA (Sequence Read Archive) database and the NCBI Nucleotide database, respectively.