Identification of QTLs associated with sweet sorghum quality
Identification of QTLs associated with sweet sorghum quality
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发表时间：
2002
期刊：
影响因子：
0.6
通讯作者：
A. Natoli;C. Gorni;F. Chegdani;P. Marsan;C. Colombi;C. Lorenzoni;A. Marocco
中科院分区：
文献类型：
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作者：
A. Natoli;C. Gorni;F. Chegdani;P. Marsan;C. Colombi;C. Lorenzoni;A. Marocco
利用EcoR Ⅰ/Mse Ⅰ和Pst Ⅰ/Mse Ⅰ限制性内切酶扩增片段长度多态性（AFLP）和单序列重复序列（SSR）技术，以2个无关甜高粱自交系（LP 2 9/1和LP 113 A）杂交的12 9个F2个体为材料，构建了甜高粱分子图谱。利用8对AFLP引物在亲本间共扩增出140条清晰的多态性片段。FcoRI/MseI和PstI/MseI引物组合分别获得平均20和32.7个多态性。103个片段按孟德尔分布，94（57）个Pst/MseI和3- EcoRI/MseI与框架图显著连锁，形成10个主要连锁群，总长度为1381.5cM。将15个已知定位在参考连锁群上的SSR标记定位到AFLP连锁图上，得到了一张包含144个标记、跨度为1659.9cM的连锁图，其中8个标记未连锁。利用该框架图对99个F3代群体进行了数量性状基因座的定位，每个群体可追溯到一个F2代植株。测定了糖度、蔗糖分、干物质产量、产糖量、开花时间、株高和低温发芽力等性状。QTL分析采用简单作图法（SIM）和复合区间作图法（CIM）。除含糖量外，其他性状均存在显著的QTL，表明对该性状有主效作用的基因在群体中不存在分离。
mplified fragment length polymorphisms (AFLPs) produced with EcoRI/MseI and PstI/MseI restriction enzymes and single sequence repeats (SSRs) were used for constructing molecular map based on 129 F 2 individuals obtained from the crossing of two unrelated sweet sorghum inbred lines (LP29/1 and LP113A). Eight pairs of primers were tested and 140 clear AFLP polymorphic fragments were amplified between the parental lines. A mean of 20 and 32.7 polymorphisms were obtained for the FcoRI/MseI and PstI/MseI primer combinations, respectively. One hundred three fragments segregated according to the Mendelian distribution and 94 (57 PstI/MseI and 3- EcoRI/MseI were significantly linked to the framework map, forming 10 major linkage groups spanning 1381.5 cM. Fifteen SSR markers with known location on reference linkage groups were mapped onto the AFLP linkage map.The comprehensive map includes 144 markers and spans 1659.9 cM, while eight markers retrained unlinked. The framework map was used to identify quantitative trait loci on 99 F 3 progenies, each tracing back to a single F 2 plant. The following traits were investigated: sugar percentage, sucrose percentage, dry matter yield, sugar yield, flowering time, plant height and germination ability at low temperatures. Simple (SIM) and composite interval mapping (CIM) were used for QTL analysis. Significant QTLs were found for all the traits investigated, except for sugar percentage, indicating that there was no segregation of genes having major effects on this trait in the population investigated.