Miniature field-flow fractionation system for analysis of blood cells.
Miniature field-flow fractionation system for analysis of blood cells.
复制标题
用于血细胞分析的微型场流分级分离系统。
作者：
V. Yue;R. Kowal;L. Neargarder;L. Bond;A. Muetterties;R. Parsons
场流分离是一种分析工具，历史上曾用于分离从分子到几微米大小的颗粒或细胞的物质。该技术可以根据场流系统的配置，通过尺寸、密度、电荷或其他物理性质来实现分离。我们开发了一个微型场流系统，用于分析19厘米长的125微米深的小通道中的细胞群。重力场是实现分离的主要场，细胞分析可在20分钟内完成。当稀释血液在该系统中分级分离时，红细胞呈现单峰。对来自几个供体（正常对照和镰状细胞性贫血患者）的血液样品进行分析，得到具有略微不同的迁移率（洗脱时间）的红细胞峰。峰值迁移率与平均细胞体积或其他标准红细胞参数不直接相关。细胞密度似乎是决定该系统细胞移动性的关键因素。
Field-flow fractionation is an analytical tool that has been historically used to separate species, ranging from molecules to particles or cells several micrometers in size. This technology can effect separation by size, density, charge, or other physical properties, depending on the configuration of the field-flow system. We have developed a miniature field-flow system to analyze cell populations in a small, 125-microns-deep channel 19 cm long. Gravity is used as the primary field to effect separation, and cell analysis is performed in < 20 min. Erythrocytes elute as a single peak when diluted blood is fractionated in this system. Analysis of blood samples from several donors (normal controls and patients with sickle cell anemia) yields erythrocyte peaks with slightly different mobilities (elution times). Peak mobility does not directly correlate with mean cell volume or other standard erythrocyte parameters. Cell density appears to be a key factor in determining cell mobility with this system.