Localization of nitric oxide synthase in the brain stem neural circuit controlling esophageal peristalsis in rats.
Localization of nitric oxide synthase in the brain stem neural circuit controlling esophageal peristalsis in rats.
复制标题
一氧化氮合酶在控制大鼠食管蠕动的脑干神经回路中的定位。
DOI：
10.1016/0016-5085(95)90062-4
复制
发表时间：
1995
期刊：
影响因子：
29.4
通讯作者：
Altschuler,SM
中科院分区：
文献类型：
--
作者：
Wiedner,EB;Bao,X;Altschuler,SM
背景/目的孤立束核的中央亚核参与食道蠕动的中央反射控制。本研究采用假狂犬病毒和烟酰胺腺嘌呤二核苷酸磷酸二磷酸酶（NADPH）组织化学结合经突触逆行道示踪的方法，确定了一氧化氮合酶在控制食管蠕动的脑干回路中的存在。方法15只大鼠中10只经食管注射病毒。60-63小时后，对脑切片进行病毒免疫荧光和NADPH组织化学处理。结果荧光神经元标记仅限于模糊核的致密结构和孤立束核的中心亚核。大多数荧光标记的中央亚核神经元NADPH染色阳性（双标记）。致密组中几乎没有双标记神经元；然而，nadph染色的末端环绕荧光标记的运动神经元。结论孤束核中央亚核的前运动神经元中存在一氧化氮合酶，支配着模糊性核致密形成的食管运动神经元。食管前运动神经元体细胞和末端NADPH染色表明，NO参与了中央亚核的神经传递以及模糊核致密形成过程中食管前运动神经元与运动神经元突触接触部位的神经传递。
Background/AimsThe central subnucleus of the nucleus of the solitary tract has been implicated in central reflex control of esophageal peristalsis. This study determined the presence of nitric oxide synthase in the brain stem circuit controlling esophageal peristalsis by combining transsynaptic retrograde tract tracing with pseudorabies virus and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH) histochemistry.MethodsVirus was injected into the esophagus of 10 of 15 rats. After 60–63 hours, brain sections were processed for viral immunofluorescence and NADPH histochemistry.ResultsFluorescent neuronal labeling was limited to the compact formation of the nucleus ambiguus and the central subnucleus of the nucleus of the solitary tract. Most fluorescence-labeled neurons in the central subnucleus stained positively for NADPH (double labeled). In the compact formation, there were almost no double-labeled neurons; however, NADPH-stained terminals surrounded fluorescence-labeled motoneurons.ConclusionsNO synthase is present in premotor neurons of the central subnucleus of the nucleus of the solitary tract that innervate esophageal motoneurons in the compact formation of the nucleus ambiguus. NADPH staining in both somata and terminals of esophageal premotor neurons suggests that NO is involved in neurotransmission in the central subnucleus and at the site of synaptic contact between esophageal premotor neurons and motoneurons in the compact formation of the nucleus ambiguus.