Detection of dentin sialoprotein in rat periodontium
Detection of dentin sialoprotein in rat periodontium
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DOI：
10.1111/j.0909-8836.2004.00110.x
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发表时间：
2004-04-01
影响因子：
1.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Baba, O;Qin, CL;Butler, WT
cDNA的克隆和序列分析表明，牙本质涎磷蛋白（DSPP）是牙本质涎蛋白（DSP）和牙本质磷蛋白（DPP）的前体。牙本质涎磷蛋白必须经过蛋白水解加工才能形成这两种细胞外基质（ECM）蛋白。大量的研究使我们得出结论，DSP（和DSPP）仅由成牙本质细胞和成牙本质细胞表达。然而，最近的观察表明分布范围更广。为了验证这一假设，我们进行了系统的研究，在大鼠第一磨牙牙根形成与免疫组化技术，使用特定的抗DSP的多克隆和单克隆抗体。我们还进行了原位杂交，使用高严格性RNA探针检测DSP转录本。免疫组化研究表明DSP不仅定位于成牙本质细胞、牙本质ECM和成牙本质细胞，而且还定位于牙槽骨、细胞牙骨质、骨细胞、牙骨质细胞及其基质中。原位杂交结果与免疫组化结果一致，显示DSP转录本在牙槽骨成骨细胞、牙周膜成纤维细胞和细胞牙骨质中的成牙骨质细胞中表达。总之，这些观察结果表明DSP参与牙周组织以及牙齿结构的形成。
Cloning and sequencing of the cDNA indicates that dentin sialophosphoprotein (DSPP) is a precursor of both dentin sialoprotein (DSP) and dentin phosphoprotein (DPP). Dentin sialophosphoprotein must be proteolytically processed to form these two extracellular matrix (ECM) proteins. Numerous studies led us to conclude that DSP (and DSPP) are exclusively expressed by odontoblasts and preameloblasts. However, recent observations suggest a wider distribution. To test this hypothesis, we conducted systematic studies on rat first molar during root formation with immunohistochemical techniques using specific anti-DSP polyclonal and monoclonal antibodies. We also performed in situ hybridization, using high-stringency RNA probes to detect DSP transcripts. Immunohistochemical studies demonstrated that DSP is not only localized in odontoblasts, dentin ECM and preameloblasts, but also in alveolar bone, cellular cementum, osteocytes, cementocytes, and their matrices. The results of in situ hybridization were consistent with those from immunohistochemistry, showing the expression of DSP transcripts in osteoblasts of alveolar bone, fibroblasts in periodontal ligament and cementoblasts in cellular cementum. Together, these observations suggest that DSP is involved in formation of the periodontium as well as tooth structures.