Higher Transport and Metabolism of Glucose in Astrocytes Compared with Neurons: A Multiphoton Study of Hippocampal and Cerebellar Tissue Slices
Higher Transport and Metabolism of Glucose in Astrocytes Compared with Neurons: A Multiphoton Study of Hippocampal and Cerebellar Tissue Slices
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DOI：
10.1093/cercor/bhs309
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发表时间：
2014-01-01
期刊：
影响因子：
3.7
通讯作者：
Deitmer, Joachim W.
中科院分区：
文献类型：
--
作者：
Jakoby, Patrick;Schmidt, Elke;Deitmer, Joachim W.
葡萄糖是大脑最重要的能量底物，其细胞分布是当前非常感兴趣的主题。我们用荧光葡萄糖探针2-脱氧-D-葡萄糖衍生物6-和2-（[N-（7-硝基苯-2-氧杂-1，3-二氮唑-4-基）氨基]-2-脱氧-D-葡萄糖）（2-NBDG）在小鼠海马和小脑急性脑片中测量葡萄糖的转运和代谢。在海马体中，6-NBDG（其不被代谢并因此指示葡萄糖转运）在富含星形胶质细胞的层中被更快地摄取（Stratum radiatum [S.r.]，Stratum oriens [S.o.]）而不是锥体细胞。可代谢的2-NBDG在S. r.和S.O.比在皮层，表明更快的葡萄糖利用率在星形胶质细胞与神经元隔室。同样，我们发现更高的摄取和温度敏感性代谢的2-NBDG在Bergmann胶质细胞相比，相邻的浦肯野神经元小脑切片。使用荧光共振能量转移纳米传感器在培养细胞中6-NBDG运输和葡萄糖运输之间的比较表明，相对于葡萄糖，神经元比星形胶质细胞更好地运输6-NBDG。这些结果表明，优先运输和代谢的葡萄糖的神经胶质细胞与神经元提出的海马和小脑由我们自己（体外）和桶皮层由Chuquet等人。（体内）比预期的更明显。
Glucose is the most important energy substrate for the brain, and its cellular distribution is a subject of great current interest. We have employed fluorescent glucose probes, the 2-deoxy-D-glucose derivates 6-and 2-([N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose) (2-NBDG), to measure transport and metabolism of glucose in acute slices of mouse hippocampus and cerebellum. In the hippocampus, 6-NBDG, which is not metabolized and hence indicates glucose transport, was taken up faster in astrocyte-rich layers (Stratum radiatum [S.r.], Stratum oriens [S.o.]) than in pyramidal cells. Metabolizable 2-NBDG showed larger signals in S.r. and S.o. than in Stratum pyramidale, suggesting faster glucose utilization rate in the astrocyte versus the neuronal compartment. Similarly, we found higher uptake and temperature-sensitive metabolism of 2-NBDG in Bergmann glia when compared with adjacent Purkinje neurons of cerebellar slices. A comparison between 6-NBDG transport and glucose transport in cultured cells using a fluorescence resonance energy transfer nanosensor showed that relative to glucose, 6-NBDG is transported better by neurons than by astrocytes. These results indicate that the preferential transport and metabolism of glucose by glial cells versus neurons proposed for the hippocampus and cerebellum by ourselves (in vitro) and for the barrel cortex by Chuquet et al. (in vivo) is more pronounced than anticipated.