ASBESTOS CONTENT OF LUNG-TISSUE, LYMPH-NODES, AND PLEURAL PLAQUES FROM FORMER SHIPYARD WORKERS
ASBESTOS CONTENT OF LUNG-TISSUE, LYMPH-NODES, AND PLEURAL PLAQUES FROM FORMER SHIPYARD WORKERS
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DOI：
10.1164/ajrccm/142.4.843
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发表时间：
1990-10-01
期刊：
影响因子：
--
通讯作者：
BIANCHI, C
中科院分区：
文献类型：
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作者：
DODSON, RF;WILLIAMS, MG;BIANCHI, C
从八名前造船厂工人的肺实质、气管淋巴结和胸膜斑块中收集尸检样本。通过改进的漂白消化技术制备来自每个相应区域的组织，并将残余物收集在0.2-μ m孔聚碳酸酯或0.22-μ m混合纤维素酯过滤器上。通过光学显微镜和透射电子显微镜分别对含铁体和未涂覆纤维进行定量。注意到每个地点的石棉负荷不同。在薄壁组织和结节中均观察到含铁小体，但在斑块中未观察到含铁小体。三名受试者的淋巴结中每克干重的含铁小体比肺实质中的多。同样，所有受试者的结节中每克无涂层纤维的数量均多于实质。在肺和肺外部位观察到角闪石和温石棉纤维，其中温石棉是斑块中的主要石棉。结节和斑块中的大多数未涂覆纤维均≤长度为5 μ m。然而，一些纤维的尺寸符合“斯坦顿假说”达到这两个领域。这些残留模式很可能反映了清除对肺负荷的影响，而不是肺外区域的最终蓄积和停滞。
Autopsy samples from eight former shipyard workers were collected from lung parenchyma, tracheal lymph nodes, and pleural plaques. The tissue from each respective area was prepared by a modified bleach digestion technique, and the residue was collected on a 0.2-.mu.m pore polycarbonate or 0.22-.mu.m mixed cellulose ester filter. Quantitation of ferruginous bodies and uncoated fibers was done by light and transmission electron microscopy, respectively. Differences in the asbestos burden were noted for each site. Ferruginous bodies were observed in both parenchyma and nodes but not in plaques. Three subjects were found to have more ferruginous bodies per gram dry weight in their lymph nodes than in their lung parenchyma. Likewise, all subjects were found to have more uncoated fibers per gram in the nodes than in the parenchyma. Amphibole and chrysotile fibers were noted in the lung and extrapulmonary sites, with chrysotile being the predominant asbestiform in plaques. The majority of the uncoated fibers in both the nodes and the plaques were .ltoreq. 5 .mu.m in length. However, some fibers with dimensions conforming to the "Stanton hypothesis" reached both areas. These residual patterns most likely reflect the impact of clearance on lung burden as opposed to the eventual accumulation and stasis in the extrapulmonary areas.