Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability
Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability
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DOI：
10.1021/ja9027023
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发表时间：
2009-09-30
影响因子：
15
通讯作者：
Deniz, Ashok A.
中科院分区：
文献类型：
--
作者：
Lemke, Edward A.;Gambin, Yann;Deniz, Ashok A.
由聚二甲基硅氧烷（PDMS）制成的微流体装置通过提供高染料光稳定性和低样品粘附来解决单分子荧光实验中的关键限制。光漂白显着减少脱氧通过气体扩散通过多孔通道瓦特。在层流鞘流中进行快速缓冲液交换，然后进行光学询问，最大限度地减少了表面与样品的接触，并允许原位添加和组合其他试剂。
A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel watts. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.