The murine Hox-2 cluster of homeo box containing genes maps distal on chromosome 11 near the tail-short (Ts) locus.
The murine Hox-2 cluster of homeo box containing genes maps distal on chromosome 11 near the tail-short (Ts) locus.
复制标题
含有基因的小鼠 Hox-2 同源框簇位于 11 号染色体远端，靠近尾部短 (Ts) 基因座。
DOI：
10.1159/000132284
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Francke,U
中科院分区：
文献类型：
--
作者：
Münke,M;Cox,DR;Jackson,IJ;Hogan,BL;Francke,U
利用体细胞杂交DNA的滤膜杂交技术，将来自小鼠重组λ-克隆（H24.1）的两个探针定位于小鼠第11号染色体（MMU）上。该序列与人类同源盒基因（HoX 2）高度同源，似乎代表先前分配给MMU 11的Hox-2簇中的两个基因之一。为了区域性地绘制Hox-2簇，我们对两个H24.1探针和一个独立分离的Hox-2探针进行了原位杂交。在所有三个实验中，放射自显影银颗粒分布相似，峰位于带11 D上。该区域包含尾短（Ts）突变的基因座，该突变导致杂合子骨骼异常和纯合子早期胚胎死亡。
Two probes derived from a mouse recombinant λ-clone (H24.1), that contains a sequence closely homologous to theDrosophila antennapediahomeo box, were mapped to mouse chromosome (MMU) 11 by filter hybridization of somatic cell hybrid DNA. This sequence is highly homologous to a human homeo box gene (HoX2) and appears to represent one of the two genes in theHox-2cluster previously assigned to MMU 11. To regionally map theHox-2cluster, we have carried out in situ hybridization of the two H24.1 probes and of an independently isolatedHox-2probe. The autoradiographic silver grain distributions were similar in all three experiments with a peak over band 11 D. This region contains the locus for the tail-short(Ts) mutation which causes skeletal abnormalities in heterozygotes and early embryonic death in homozygotes.