Stress effects on AVT and CRF systems in two strains of rainbow trout (Oncorhynchus mykiss) divergent in stress responsiveness
Stress effects on AVT and CRF systems in two strains of rainbow trout (Oncorhynchus mykiss) divergent in stress responsiveness
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DOI：
10.1016/j.yhbeh.2010.11.008
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发表时间：
2011-01-01
影响因子：
3.5
通讯作者：
Winberg, Svante
中科院分区：
文献类型：
--
作者：
Backstrom, Tobias;Schjolden, Joachim;Winberg, Svante
本研究的目的是研究两个品系虹鱼F4代的血浆皮质醇对限制应激(HR：高应答者或LR：低应答者)的反应是否也会在应激诱导的促肾上腺皮质激素释放因子(CRF)mRNA浓度方面有所不同。精氨酸催产素(AVT)。CRF受体1型(CRF-R1)。CRF受体2型(CRF-R2)和AVT受体(AVT-R)。此外，还监测了血浆皮质醇浓度、单胺和单胺代谢物的脑干水平以及监禁期间的行为。结果证实，HR和LR鲑鱼对禁闭的皮质醇反应不同，并表明这两个品系的鱼对禁闭的行为反应也不同。在禁闭期间，HR鲑鱼表现出显著高于LR鲑鱼的运动活动。此外，在禁闭180分钟后，HR鱼的前脑CRF mRNA浓度显著高于LR鱼。此外，当受到30分钟的限制时，HR FISH的CRF-R2 mRNA浓度显著低于LR FISH，而CRF-R1mRNA的浓度则没有差异。在束缚30min和180min时，LR和HR FISH之间的AVT或AVT-R基因表达。CRF和CRF-R2基因表达的差异可能与不同虹鱼品系在应激应对上的差异有关。(C)2010 Elsevier Inc.保留所有权利。
The aim for this study was to examine whether the F4 generation of two strains of rainbow trout divergent in their plasma cortisol response to confinement stress (HR: high responder or LR: low responder) would also differ in stress-induced effects on forebrain concentrations of mRNA for corticotropin-releasing factor (CRF). arginine vasotocin (AVT). CRF receptor type 1 (CRF-R1). CRF receptor type 2 (CRF-R2) and AVT receptor (AVT-R). In addition, plasma cortisol concentrations, brainstem levels of monoamines and monoamine metabolites, and behaviour during confinement were monitored. The results confirm that HR and LR trout differ in their cortisol response to confinement and show that fish of these strains also differ in their behavioural response to confinement. The HR trout displayed significantly higher locomotor activity while in confinement than LR trout. Moreover, following 180 min of confinement HR fish showed significantly higher forebrain concentrations of CRF mRNA than LR fish. Also, when subjected to 30 min of confinement HR fish showed significantly lower CRF-R2 mRNA concentrations than LR fish, whereas there were no differences in CRF-R1. AVT or AVT-R mRNA expression between LR and HR fish either at 30 or 180 min of confinement. Differences in the expression of CRF and CRF-R2 mRNA may be related to the divergence in stress coping displayed by these rainbow trout strains. (C) 2010 Elsevier Inc. All rights reserved.