ANTIMETASTATIC EFFECTS ASSOCIATED WITH PLATELET REDUCTION
ANTIMETASTATIC EFFECTS ASSOCIATED WITH PLATELET REDUCTION
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DOI：
10.1073/pnas.61.1.46
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发表时间：
1968-01-01
影响因子：
11.1
通讯作者：
STEWART, CC
中科院分区：
文献类型：
--
作者：
GASIC, GJ;GASIC, TB;STEWART, CC
我们以前报道过，如果小鼠首先注射神经氨酸酶，TA3腹水肿瘤细胞接种小鼠后引起的转移数量可以减少50%。据推测，这种效应可能反映了神经氨酸酶对肿瘤或宿主内皮细胞唾液酸外壳的作用，因为人们认为这些细胞类型之间的相互黏性在转移形成中很重要。由于神经氨酸酶在注射到宿主之前剥去肿瘤细胞的包膜似乎没有什么抗转移作用，因此我们将注意力集中在神经氨酸酶对宿主的作用上。本文报道了神经氨酸酶在体内产生血小板减少的意外发现，血小板水平的降低与转移的减少密切相关。在进一步的实验中，其他药物改变了循环血小板的浓度，证实了血小板水平与转移之间的关联，并提示先前观察到的神经氨酸酶的抗转移作用可能至少部分是由其对血小板的作用介导的。材料和方法。在CAFi/Jax雌性小鼠腹腔培养-TA3腹水肿瘤细胞s2，每只小鼠分别接受2.0-2.5 × 105或3.0-3.5 × 105肿瘤细胞悬悬于0.2 ml培养基中。14 ~ 16 d后处死，计数乙醚和肺转移灶。
We have previously reported that the number of metastases resulting from the inoculation of TA3 ascites tumor cells into mice could be reduced by 50 per cent if the mice were first injected with neuraminidase.'It was assumed that this effect probably reflected the action of neuraminidase on the sialic acid coat of tumor or host endothelial cells, since it was believed that mutual stickiness be-tween these cell types was important in metastasis formation.'Since the uncoating of tumor cells by neuraminidase prior to injection into the host seemed to have very little antimetastatic effect,'attention was directed to the effects of neuraminidase on the host. The present paper reports the unexpected finding that neuraminidase in vivo produces thrombocytopenia, with a close correspondence between the decreasein platelet levels and the decrease in metastases. Further experiments, in which the concentration of circulating platelets was varied by other agents, confirm the association between platelet levels and metastases, and suggest that the previously observed antimetastatic effect of neuraminidase may be at least partially mediated by its effect on the platelets.Material8 and Methods.-TA3 ascites tumor cells2 were grown in the peritoneal cavity of CAFi/Jax female mice, each receiving 2.0-2.5 X 105 or 3.0-3.5 X 105 tumor cells sus-pended in 0.2 ml of medium. They were killed 14-16 days later, and ether and lung metastases were counted.