Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells.
Wnt and Frizzled RNA expression in human mesenchymal and embryonic (H7) stem cells.
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DOI：
10.1186/1750-2187-3-16
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发表时间：
2008-09-26
影响因子：
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通讯作者：
Wang, Hsien-Yu
中科院分区：
文献类型：
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作者：
Okoye, Ujunwa C;Malbon, Craig C;Wang, Hsien-Yu
背景：Wnt信号在胚胎干细胞的更新、生长和分化中起重要作用。虽然19 Wnt，10 Frizzled基因已被确定在哺乳动物中，他们在干细胞中的表达模式在很大程度上是未知的。结果：我们进行了RNA表达谱的Wnt配体，其细胞受体“Frizzleds”和共同受体LRP 5/6在人胚胎干细胞（H7），人骨髓间充质细胞，以及小鼠全能F9畸胎瘤胚胎细胞。除Wnt 2基因不表达外，全能F9细胞表达所有其他18个Wnt基因以及Frizzled基因家族的所有10个成员的RNA。H7细胞表达19个Wnt基因中每一个的RNA。相比之下，人类间充质细胞未显示可检测的Wnt 1、Wnt 8a、Wnt 8b、Wnt 9 b、Wnt 10a和Wnt 11的RNA表达。在H7和人类间质细胞中的卷曲RNA的分析揭示了受体基因家族的9个成员的表达，除了卷曲8。在所有三种细胞系中检测到卷曲共受体LRP 5和LRP 6基因的表达。人H7和小鼠F9细胞表达几乎完全互补的Wnts和Frizzleds基因。而人骨髓间充质细胞中Wnt 1、8a、8b、9 b、10a和11等6种配体均不表达。结论：人H7和小鼠F9胚胎细胞表达大部分Wnt和Frizzleds基因。相反，多能间充质细胞缺乏Frizzled-8和6 Wnt基因的表达。
BACKGROUND: Wnt signals are important for embryonic stem cells renewal, growth and differentiation. Although 19 Wnt, 10 Frizzled genes have been identified in mammals, their expression patterns in stem cells were largely unknown.RESULTS: We conducted RNA expression profiling for the Wnt ligands, their cellular receptors "Frizzleds" and co-receptors LRP5/6 in human embryonic stem cells (H7), human bone marrow mesenchymal cells, as well as mouse totipotent F9 teratocarcinoma embryonal cells. Except failing to express Wnt2 gene, totipotent F9 cells expressed RNA for all other 18 Wnt genes as well as all 10 members of Frizzled gene family. H7 cells expressed RNA for each of the 19 Wnt genes. In contrast, human mesenchymal cells did not display detectable RNA expression of Wnt1, Wnt8a, Wnt8b, Wnt9b, Wnt10a, and Wnt11. Analysis of Frizzled RNAs in H7 and human mesechymal cells revealed expression of 9 members of the receptor gene family, except Frizzled8. Expression of the Frizzled co-receptor LRP5 and LRP6 genes were detected in all three cell lines. Human H7 and mouse F9 cells express nearly a full complement of both Wnts and Frizzleds genes. The human mesenchymal cells, in contrast, have lost the expression of six Wnt ligands, i.e. Wnt1, 8a, 8b, 9b, 10a and 11.CONCLUSION: Puripotent human H7 and mouse F9 embryonal cells express the genes for most of the Wnts and Frizzleds. In contrast, multipotent human mesenchymal cells are deficient in expression of Frizzled-8 and of 6 Wnt genes.