P2X7 mRNA expression in non-small cell lung cancer: MicroRNA regulation and prognostic value.
P2X7 mRNA expression in non-small cell lung cancer: MicroRNA regulation and prognostic value.
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DOI：
10.3892/ol.2014.2620
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发表时间：
2015-01
期刊：
影响因子：
2.9
通讯作者：
Fontanini G
中科院分区：
文献类型：
--
作者：
Boldrini L;Giordano M;Alì G;Melfi F;Romano G;Lucchi M;Fontanini G
人类 P2X7 受体很重要，并且在肿瘤形成中表现出多种功能。目前，人们对其监管知之甚少。 P2X7 表达可能在转录后受到调节，并且推定的 microRNA (miRNA) 结合位点被认为参与其中。本研究的目的是确定 miRNA（miR-21、let-7 g 和 miR-205）是否调节 P2X7 mRNA 稳定性。此外，还研究了 P2X7 表达对非小细胞肺癌 (NSCLC) 患者的影响。使用定量逆转录聚合酶链反应对 96 例 NSCLC 病例中的 P2X7 mRNA 和成熟 Let-7g、miR-21 和 miR-205 表达水平进行定量。在所有样本中，还进行了表皮生长因子受体和 K-Ras 突变分析。与 P2X7 高表达的样品相比，P2X7 低表达的样品在 miR-21 表达方面表现出更高的倍数变化。与 K-Ras 野生型肿瘤患者相比，K-Ras 突变 NSCLC 患者的肿瘤中 miR-21 表达显着升高（P=0.003）。此外，为了评估 NSCLC 患者中 P2X7 表达与预后之间的关联，使用 Kaplan-Meier 方法进行生存分析。 P2X7 高表达的 NSCLC 患者与低表达的患者相比，无进展生存期和总生存期存在显着差异（分别为 P=0.03 和 P=0.02）。因此，我们假设携带 K-Ras 突变的 NSCLC 患者中 miR-21 的高水平表达可能受到复杂回路的调节，包括 P2X7 下调，这些过程共同可能促进肿瘤进展。
The human P2X7 receptor is significant and exhibits several functions in neoplasia. At present, little is known with regard to its regulation. P2X7 expression may be regulated post-transcriptionally and putative microRNA (miRNA) binding sites are considered to be involved. The aim of this study was to determine whether miRNAs (miR-21, let-7 g and miR-205) regulate P2X7 mRNA stability. In addition, the impact of P2X7 expression in patients with non-small cell lung cancer (NSCLC) was investigated. P2X7 mRNA and mature Let-7 g, miR-21, and miR-205 expression levels were quantified in 96 NSCLC cases using quantitative reverse transcription polymerase chain reaction. In all samples, epidermal growth factor receptor and K-Ras mutational analysis was also performed. Samples with low P2X7 expression were found to exhibit a higher fold change in miR-21 expression when compared with samples exhibiting high P2X7 expression. Significantly higher miR-21 expression was observed in the tumors of NSCLC patients with a K-Ras mutation when compared with patients who had K-Ras wild-type tumors (P=0.003). Additionally, to evaluate the association between P2X7 expression and prognosis in NSCLC patients, survival analysis was performed using the Kaplan-Meier method. A significant difference in the progression-free survival and overall survival in the NSCLC patients with high P2X7 expression was identified, when compared with that of patients with low expression (P=0.03 and P=0.02, respetively). Therefore, we hypothesized that high levels of miR-21 expression in NSCLC patients with K-Ras mutations may be regulated by a complex circuit, including P2X7 downregulation and together these processes may promote tumor progression.
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影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
影响因子：
4.2
作者：
Capodanno A;Boldrini L;Alì G;Pelliccioni S;Mussi A;Fontanini G
通讯作者：
Fontanini G
影响因子：
4.8
作者：
Zhou, Lingyin;Qi, Xiaoping;Gorodeski, George I.
通讯作者：
Gorodeski, George I.
影响因子：
2.9
作者：
Rahman, Omar Abdul;Sasvari-Szekely, Maria;Nemoda, Zsofia
通讯作者：
Nemoda, Zsofia
DOI：
10.1097/jto.0b013e318206a221
发表时间：
2011-02
期刊：
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
影响因子：
--
作者：
Travis WD;Brambilla E;Noguchi M;Nicholson AG;Geisinger KR;Yatabe Y;Beer DG;Powell CA;Riely GJ;Van Schil PE;Garg K;Austin JH;Asamura H;Rusch VW;Hirsch FR;Scagliotti G;Mitsudomi T;Huber RM;Ishikawa Y;Jett J;Sanchez-Cespedes M;Sculier JP;Takahashi T;Tsuboi M;Vansteenkiste J;Wistuba I;Yang PC;Aberle D;Brambilla C;Flieder D;Franklin W;Gazdar A;Gould M;Hasleton P;Henderson D;Johnson B;Johnson D;Kerr K;Kuriyama K;Lee JS;Miller VA;Petersen I;Roggli V;Rosell R;Saijo N;Thunnissen E;Tsao M;Yankelewitz D
通讯作者：
Yankelewitz D