Genetic Analysis and Molecular Mapping of a Novel Chlorophyll-Deficit Mutant Gene in Rice
Genetic Analysis and Molecular Mapping of a Novel Chlorophyll-Deficit Mutant Gene in Rice
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DOI：
10.1016/s1672-6308(08)60013-x
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发表时间：
2008-03-01
期刊：
影响因子：
4.8
通讯作者：
Deng, Xiao-jian
中科院分区：
文献类型：
--
作者：
Huang, Xiao-qun;Wang, Ping-rong;Deng, Xiao-jian
从一个正常的绿色水稻品种824B中鉴定出一个叶绿素缺乏的水稻黄化突变体824ys。从苗期开始，植株整体呈黄绿色，分蘖数减少，生育期延长。突变体叶片叶绿素、叶绿素a、叶绿素b含量、净光合速率、每穗颖花数、结实率和千粒重均明显低于野生型亲本。对824ys的F-1和F-2代与3个正常绿色品种杂交的遗传分析表明，其叶绿素亏缺突变性状受一对隐性核基因控制。利用微卫星标记和495R/824ys F2定位群体对突变基因进行遗传定位，将824ys突变基因定位在水稻3号染色体短臂上。目标基因与标记RM218、RM282和RM6959的遗传距离分别为25.6 cM、5.2 cM和21.8 cM。它被认为是一个新的叶绿素缺陷突变基因，暂定名为chl11(t)。
A rice etiolation mutant 824ys featured with chlorophyll deficiency was identified from a normal green rice variety 824B. It showed whole green-yellow plant from the seedling stage, reduced number of tillers and longer growth duration. The contents of chlorophyll, chlorophyll a, chlorophyll b and net photosynthetic rate in leaves of the mutant obviously decreased, as well as the number of spikelets per panicle, seed setting rate and 1000-grain weight compared with its wild-type parent. Genetic analyses on F-1 and F-2 generations of 824ys crossed with three normal green varieties showed that the chlorophyll-deficit mutant character was controlled by a pair of recessive nuclear gene. Genetic mapping of the mutant gene was conducted by using microsatellite markers and F2 mapping population of 495R/824ys, and the mutant gene of 824ys was mapped on the short arm of rice chromosome 3. The genetic distances from the target gene to the markers RM218, RM282 and RM6959 were 25.6 cM, 5.2 cM and 21.8 cM, respectively. It was considered to be a new chlorophyll-deficit mutant gene and tentatively named as chl11(t).