Active actin gels
Active actin gels
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活性肌动蛋白凝胶
DOI：
10.4161/cib.18286
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发表时间：
2012
影响因子：
--
通讯作者：
J. Molloy
中科院分区：
文献类型：
--
作者：
Shahid M. Khan;R. Ali;N. Asi;J. Molloy
肌动蛋白细丝的自组织是一个将细胞生物学与凝聚态物理学联系在一起的话题。在体外测试中，可以精确地操纵组件的机械和化学性能，这是对理论模型进行严格测试所必需的。我们回顾了最近在体外运动性分析方面的工作，这些工作记录了在高肌丝密度下由肌球蛋白马达蛋白驱动的有序肌动蛋白微丝微域的出现。马达和灯丝表面密度和机械力化学循环动力学是目前研究的其他参数。为了解释突然出现的群体行为，已经研究了个体细丝碰撞。在高电机密度下，由交叉事件中的局部细丝变形所证明的非极性、弱相互作用被衰减。理论分析需要对刚性杆丝模型进行修正。在完整的细胞中，辅助蛋白调节肌动蛋白细丝的长度、束或滑动，这导致了复杂的突现结构和行为，如细胞运动和趋化。开发具有预测能力的通用、机械和生化框架，将分子特性与活细胞中看到的微观和宏观现象联系起来，需要理论家和实验学家之间的对话。
The self-organization of actin filaments is a topic that links cell biology with condensed matter physics. In vitro assays allow precise manipulation of component mechanical and chemical properties, needed for rigorous tests of theoretical models. We review recent work on in vitro motility assays that documented emergence of ordered actin filament microdomains powered by myosin motor proteins at high filament densities. Motor and filament surface density and mechanochemical cycle kinetics are additional parameters under current investigation. Individual filament collisions have been studied in order to elucidate the emergent population behavior. Apolar, weak interactions evidenced by local filament deformations during crossover events are attenuated at high motor densities. Theoretical analysis requires refinement of rigid rod filament models. In intact cells, accessory proteins modulate actin filament length, bundling or sliding and this gives rise to complex emergent structures and behaviors such as cell motility and chemotaxis. The development of generic, mechanical and biochemical frameworks with predictive power that link molecular properties with micro- and macroscopic phenomena seen in living cells requires dialogue between theoreticians and experimentalists.
DOI：
10.1073/pnas.83.17.6272
发表时间：
1986-09-01
影响因子：
11.1
作者：
KRON, SJ;SPUDICH, JA
通讯作者：
SPUDICH, JA