Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus
Mild alkali-pretreatment effectively extracts guaiacyl-rich lignin for high lignocellulose digestibility coupled with largely diminishing yeast fermentation inhibitors in Miscanthus
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DOI：
10.1016/j.biortech.2014.07.017
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发表时间：
2014-10-01
影响因子：
11.4
通讯作者：
Peng, Liangcai
中科院分区：
文献类型：
--
作者：
Li, Ming;Si, Shengli;Peng, Liangcai
本研究以三对芒属植物材料为材料，对不同碱预处理的木质纤维素酶解物进行了评价，结果表明，芒属植物具有三种不同的单醇(G、S、H)组成。与酶解得到的配对样品相比，G-水平升高的Mfl26样品的己糖产量显著增加了1.61倍，而高H-水平的Msa29样品的己糖产量仅增加了1.32倍。相比之下，S水平升高的mfl30样品显示，与p<；0.01的配对样品0.89-0.98倍相比，己糖产量降低。值得注意的是，只有富含G的生物质样品在4%NaOH预处理下表现出完全的酶水解。此外，富含G的样品比S和富H的样品更能有效地提取木质素-半纤维素复合体，导致大量木质素抑制剂被去除到酵母发酵中。因此，本研究提出了一种对芒属植物进行微小的遗传木质素修饰以实现低成本生物质生产的最优途径。(C)2014爱思唯尔有限公司。保留所有权利。
In this study, various alkali-pretreated lignocellulose enzymatic hydrolyses were evaluated by using three standard pairs of Miscanthus accessions that showed three distinct monolignol (G, S, H) compositions. Mfl26 samples with elevated G-levels exhibited significantly increased hexose yields of up to 1.61-fold compared to paired samples derived from enzymatic hydrolysis, whereas Msa29 samples with high H-levels displayed increased hexose yields of only up to 1.32-fold. In contrast, Mfl30 samples with elevated S-levels showed reduced hexose yields compared to the paired sample of 0.89-0.98 folds at p < 0.01. Notably, only the G-rich biomass samples exhibited complete enzymatic hydrolysis under 4% NaOH pretreatment. Furthermore, the G-rich samples showed more effective extraction of lignin-hemicellulose complexes than the S- and H-rich samples upon NaOH pretreatment, resulting in large removal of lignin inhibitors to yeast fermentation. Therefore, this study proposes an optimal approach for minor genetic lignin modification towards cost-effective biomass process in Miscanthus. (C) 2014 Elsevier Ltd. All rights reserved.