IMMUNOHISTOCHEMICAL LOCALIZATION OF CARBAMOYL-PHOSPHATE SYNTHETASE (AMMONIA) IN ADULT-RAT LIVER - EVIDENCE FOR A HETEROGENEOUS DISTRIBUTION
IMMUNOHISTOCHEMICAL LOCALIZATION OF CARBAMOYL-PHOSPHATE SYNTHETASE (AMMONIA) IN ADULT-RAT LIVER - EVIDENCE FOR A HETEROGENEOUS DISTRIBUTION
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DOI：
10.1177/32.6.6373912
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发表时间：
1984-01-01
影响因子：
3.2
通讯作者：
CHARLES, R
中科院分区：
文献类型：
--
作者：
JANZEN, JWG;LAMERS, WH;CHARLES, R
比较不同的固定介质，以找到一种保存大鼠肝脏的组织结构，并允许明确的免疫组织化学检测氨甲酰磷酸合成酶（氨）。将大鼠肝脏固定在甲醇、丙酮和水的混合物中，产生最强烈的免疫组织化学染色。采用针对大鼠肝磷酸氨基甲酸酯合成酶的特异性抗血清，固定后酶蛋白提取率< 1%，组织结构与Bouin固定液固定后相似。特异性免疫组织化学染色仅局限于实质细胞的细胞质；其颗粒状分布与氨甲酰磷酸合成酶的线粒体定位一致。免疫组化染色显示肝腺泡内分布不均。门静脉周围染色最强烈，向肝小静脉缓慢减弱，在肝小静脉周围的有限区域，染色突然减弱后几乎不存在。讨论了氨甲酰磷酸合成酶的不均匀分布对氨代谢的可能意义。
Different fixation media were compared in order to find one that preserves the histological structure of rat liver and allows unambiguous immunohistochemical detection of carbamoyl-phosphate synthetase (ammonia). Fixation of rat liver in a mixture of methanol, acetone and water yields the most intense immunohistochemical staining. Using a specific antiserum raised against rat liver carbamoly-phosphate synthetase, < 1% of the enzyme protien is extractable after this fixation procedure, and the histological structure is similar to that after fixation in Bouin''s fixative. Specific immunohistochemical staining is localized exclusively in the cytoplasm of the parenchymal cells; its granular distribution is in accordance with the mitochondrial localization of carbamoyl-phosphate synthetase. Immunohistochemical staining shows a heterogeneous distribution within the liver acinus. Staining is most intense around the portal venules, decreases slowly toward the hepatic venules and is, after an abrupt decrease, virtually absent in a limited area surrounding these venules. The possible significance of the heterogeneous distribution of carbamoyl-phosphate synthetase for ammonia metabolism is discussed.