Enhanced expression of annexin II in human pancreatic carcinoma cells and primary pancreatic cancers.
Enhanced expression of annexin II in human pancreatic carcinoma cells and primary pancreatic cancers.
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DOI：
10.1093/carcin/14.12.2575
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发表时间：
1993-12
期刊：
影响因子：
4.7
通讯作者：
J. Vishwanatha;Y. Chiang;K. Kumble;K. Kumble;M. Hollingsworth;P. Pour
中科院分区：
文献类型：
--
作者：
J. Vishwanatha;Y. Chiang;K. Kumble;K. Kumble;M. Hollingsworth;P. Pour
膜联蛋白II是一种钙和磷脂结合蛋白，是蛋白酪氨酸激酶的底物。最近的研究发现膜联蛋白II参与DNA合成和细胞增殖。在癌细胞和组织中观察到膜联蛋白II水平升高。为了研究膜联蛋白II在胰腺腺癌细胞和原发肿瘤中的表达，我们测定了正常胰腺、5个已建立的人胰腺腺癌细胞系、3个原发胰腺癌和1个转移瘤中膜联蛋白II mRNA和蛋白的表达水平。与正常胰腺相比，所有5种细胞系的膜联蛋白II水平都高出5至15倍。在三个原发性胰腺肿瘤和一个转移性肿瘤中观察到膜联蛋白II表达显著升高（2- 8倍）。免疫细胞化学分析表明，膜联蛋白II的表达增加仅限于增殖的导管腺癌，并且膜联蛋白II的表达与表达PCNA的细胞共定位。在正常胰腺中，膜联蛋白II在导管细胞和导管细胞中表达，在腺泡细胞和胰岛细胞中未见表达。我们从这些发现中得出结论，膜联蛋白II在细胞增殖中起作用，其调节在胰腺癌中发生改变。
Annexin II is a calcium and phospholipid binding protein and a substrate for protein-tyrosine kinases. Recent investigations have revealed involvement of annexin II in DNA synthesis and cell proliferation. Increased levels of annexin II are observed in cancer cells and tissues. To investigate the expression of annexin II in pancreatic adenocarcinoma cells and primary tumors, we measured the levels of annexin II mRNA and protein in normal human pancreas, five established human pancreatic adenocarcinoma cell lines, three primary pancreatic cancers and one metastatic tumor. All five cell lines examined had 5- to 15-fold higher levels of annexin II as compared to normal pancreas. Significant elevations (2- to 8-fold) of annexin II expression were observed in the three primary pancreatic tumors and one metastatic tumor examined. Immunocytochemical analysis indicates that the increased expression of annexin II is limited to proliferating ductular adenocarcinoma, and annexin II expression co-localizes with cells that express PCNA. In normal pancreas, annexin II expression is seen in ductal and ductular cells and no expression is seen in acinar or islet cells. We conclude from these findings that annexin II has a role in cell proliferation and its regulation is altered in pancreatic cancer.