Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice
Intranasal mite allergen induces allergic asthma-like responses in NC/Nga mice
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DOI：
10.1016/j.lfs.2005.06.020
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发表时间：
2006-01-25
期刊：
影响因子：
6.1
通讯作者：
Ishiyama, H
中科院分区：
文献类型：
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作者：
Shibamori, M;Ogino, K;Ishiyama, H
在NC/Nga小鼠中研究了鼻内施用螨变应原而不含佐剂诱导的气道反应。粉尘螨粗提物（Dermatophagoides farinae）连续给药5天，末次致敏后1周给予单次鼻内激发加强剂量。单次激发后24 h，测量气道高反应性（AHR），并分析支气管肺泡灌洗液（BALF）中嗜酸性粒细胞和中性粒细胞的数量以及细胞因子和趋化因子水平。Df暴露后BALF中嗜酸性粒细胞数量、AHR、Th 2细胞因子（IL-5和IL-13）和趋化因子（eotaxin-1和eotaxin-2）水平显著增加。C57 BL/6 N、A/J、BALB/c和CBA/JN小鼠品系也暴露于Df粗提物，但所有测量的反应在NC/Nga小鼠中最强。此外，DF暴露的NC/Nga小鼠表现出杯状细胞增生，肺嗜酸性粒细胞炎症，血清总IgE和DF特异性IgG 1增加。NC/Nga小鼠鼻内暴露于屋尘螨粗提物后，BALF嗜酸性粒细胞增多和AHR与DE诱导的反应相似。鼻内暴露于卵清蛋白后，研究参数均未增加。这些数据表明，NC/Nga小鼠鼻内暴露于螨过敏原后发生过敏性哮喘样反应。(c)2005年爱思唯尔公司All rights reserved.
Airway responses induced by intranasal administration of mite allergen without adjuvant were studied in NC/Nga mice. A crude extract of Dermatophagoides farinae (Do was administered for 5 consecutive days and a single intranasal challenge booster dose was given 1 week after the last sensitization. 24 h after the single challenge, the airway hyperresponsiveness (AHR) was measured and the bronchoalveolar lavage fluid (BALF) was analyzed for numbers of eosinophils and neutrophils, and both cytokine and chemokine levels. There were marked increases in number of eosinophils in the BALF, AHR, Th2 cytokines (IL-5 and IL-13), and chemokine (eotaxin-1 and eotaxin-2) levels in the BALF following Df exposure. C57BL/6N, A/J, BALB/c, and CBA/JN mouse strains were also exposed to Df crude extract, but all of the measured responses were strongest in NC/Nga mice. Furthermore, Df-exposed NC/Nga mice showed the goblet cell hyperplasia, pulmonary eosinophilic inflammation, and increases in both total serum IgE and Df-specific IgG1. After intranasal exposure of NC/Nga mice to crude extract of Dermatophagoides pteronyssinus, the BALF eosinophilia and AHR were similar to responses induced by DE None of the study parameters were increased in response to intranasal exposure to ovalbumin. These data demonstrated that NC/Nga mice developed allergic asthma-like responses after intranasal exposure to mite allergens. (c) 2005 Elsevier Inc. All rights reserved.