Gibberellin Signaling Is Required for Far-Red Light-Induced Shoot Elongation in Pinus tabuliformis Seedlings
Gibberellin Signaling Is Required for Far-Red Light-Induced Shoot Elongation in Pinus tabuliformis Seedlings
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远红光诱导油松幼苗芽伸长需要赤霉素信号传导
DOI：
10.1104/pp.19.00954
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发表时间：
2020-01-01
期刊：
影响因子：
7.4
通讯作者：
Niu, Shi-Hui
中科院分区：
文献类型：
--
作者：
Li, Wei;Liu, Shuang-Wei;Niu, Shi-Hui
赤霉素(GA)在弱红/远红(R：FR)光比调控下胚轴和叶柄伸长中起重要作用。然而，低R：FR和GAS之间的调控关系仍不清楚，特别是在裸子植物中。为了加深对低R：FR介导的松树新梢伸长的分子基础的了解，并确定低R：FR与GAs作用之间是否存在关联，我们研究了低R：FR、GAs和GAs生物合成抑制剂多效唑(PAC)对油松幼苗的形态和转录的影响。转录组图谱显示，低R：FR条件和GAS在油松中有一组共同的转录靶点。我们提供的证据表明，低R：FR对油松新梢伸长的影响至少部分受气体积累的调制，而气体积累可以在很大程度上被PAC减弱。在低R：FR反应中，GAs也参与了不同植物激素之间的串扰。编码内生酸氧化酶(Kao)的GA生物合成基因被低R：FR强烈激活，而不受气体反馈调节和光周期的影响。结果表明，低R：FR诱导油松幼苗芽伸长需要GA信号，针叶植物和被子植物对低R：FR介导的GA生物合成有不同的调控靶点。
Gibberellin (GA) is known to play an important role in low red/far-red (R:FR) light ratio-mediated hypocotyl and petiole elongation in Arabidopsis (Arabidopsis thaliana). However, the regulatory relationship between low R:FR and GAs remains unclear, especially in gymnosperms. To increase our understanding of the molecular basis of low R:FR-mediated shoot elongation in pines and to determine whether there is an association between low R:FR and GAs action, we explored the morphological and transcriptomic changes triggered by low R:FR, GAs, and paclobutrazol (PAC), a GAs biosynthesis inhibitor, in Pinus tabuliformis seedlings. Transcriptome profiles revealed that low R:FR conditions and GAs have a common set of transcriptional targets in P. tabuliformis. We provide evidence that the effect of low R:FR on shoot elongation in P. tabuliformis is at least partially modulated by GAs accumulation, which can be largely attenuated by PAC. GAs are also involved in the cross talk between different phytohormones in the low R:FR response. A GA biosynthesis gene, encoding entkaurenoic acid oxidase (KAO), was strongly stimulated by low R:FR without being affected by GAs feedback regulation or the photoperiod. We show that GA signaling is required for low R:FR-induced shoot elongation in P. tabuliformis seedlings, and that there are different regulatory targets for low R:FR-mediated GA biosynthesis between conifers and angiosperms.