Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain
Isolation and characterization of SATB2, a novel AT-rich DNA binding protein expressed in development- and cell-specific manner in the rat brain
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DOI：
10.1007/s11064-005-9012-8
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发表时间：
2006-02-01
影响因子：
4.4
通讯作者：
Agoston, DV
中科院分区：
文献类型：
--
作者：
Szemes, M;Gyorgy, A;Agoston, DV
富含AT的DNA元件在调节细胞特异性基因表达方面起着重要作用。作为一种富含AT的DNA结合蛋白，SATB1是一种新型的转录因子，通过染色质修饰来调节基因在造血系中的表达。利用DNA亲和纯化和质谱法，我们从发育中的大鼠大脑皮层中鉴定和分离了一个相关蛋白SATB2。SATB2与SATB1同源，大鼠的蛋白与小鼠和人的SATB2几乎相同。利用竞争性EMSA，我们发现重组SATB2蛋白与富含AT的dsDNA具有很高的亲和力和特异性。利用RT-PCR、Western分析和免疫组织化学方法，我们证明SATB2的表达仅限于E16和P4龄大鼠新皮质中有丝分裂后、分化的神经元的一部分。我们认为，与其同系物SATB1类似，SATB2也通过改变皮质神经元分化过程中的染色质结构来调节基因的表达。
AT-rich DNA elements play an important role in regulating cell-specific gene expression. One of the AT-rich DNA binding proteins, SATB1 is a novel type of transcription factor that regulates gene expression in the hematopoietic lineage through chromatin modification. Using DNA-affinity purification followed by mass spectrometry we identified and isolated a related protein, SATB2 from the developing rat cerebral cortex. SATB2 shows homology to SATB1 and the rat protein is practically identical to the mouse and human SATB2. Using competitive EMSA, we show that recombinant SATB2 protein binds with high affinity and specificity to AT-rich dsDNA. Using RT-PCR, Western analysis and immunohistochemistry we demonstrate that SATB2 expression is restricted to a subset of postmitotic, differentiating neurons in the rat neocortex at ages E16 and P4. We suggest that similar to its homologue SATB1, SATB2 is also involved in regulating gene expression through altering chromatin structure in differentiating cortical neurons.