Sensitive Method for Reliable Quantification of Sulfane Sulfur in Biological Samples
Sensitive Method for Reliable Quantification of Sulfane Sulfur in Biological Samples
复制标题
生物样品中硫烷硫的可靠定量的灵敏方法
DOI：
10.1021/acs.analchem.9b02875
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发表时间：
2019-09-17
影响因子：
7.4
通讯作者：
Xun, Luying
中科院分区：
文献类型：
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作者：
Ran, Mingxue;Wang, Tianqi;Xun, Luying
磺胺硫已被认为是一种常见的细胞成分，参与调节酶活性和信号通路。然而，生物样品中总砜硫的定量仍然是一个挑战。在这里，我们开发了一种方法来解决这个需求。在pH为9.5的溶液中，在95℃下加热10分钟，所有被测的磺胺硫与亚硫酸盐反应并定量转化为硫代硫酸盐。该分析条件也足以将生物样品中的总砜硫转化为硫代硫酸盐，以便进一步衍生化和定量。应用该方法测定了细菌、酵母、哺乳动物细胞和斑马鱼不同生长阶段的硫含量。总磺胺硫含量在生长初期均呈上升趋势，在生长后期保持稳定状态，并急剧下降。不同树种的硫含量不同。对于大肠杆菌，培养基对其硫的含量也有影响。在小鼠和虾的不同器官中也检测到总磺胺硫含量，从1 ~ 10 nmol/（mg蛋白质）不等。因此，该方法适用于生物样品中总砜硫的定量分析。
Sulfane sulfur has been recognized as a common cellular component, participating in regulating enzyme activities and signaling pathways. However, the quantification of total sulfane sulfur in biological samples is still a challenge. Here, we developed a method to address the need. All tested sulfane sulfur reacted with sulfite and quantitatively converted to thiosulfate when heated at 95 degrees C in a solution of pH 9.5 for 10 min. The assay condition was also sufficient to convert total sulfane sulfur in biological samples to thiosulfate for further derivatization and quantification. We applied the method to detect sulfane sulfur contents at different growth phases of bacteria, yeast, mammalian cells, and zebrafish. Total sulfane sulfur contents in all of them increased in the early stage, kept at a steady state for a period, and declined sharply in the late stage of the growth. Sulfane sulfur contents varied in different species. For Escherichia coli, growth media also affected the sulfane sulfur contents. Total sulfane sulfur contents from different organs of mouse and shrimp were also detected, varying from 1 to 10 nmol/(mg of protein). Thus, the new method is suitable for the quantification of total sulfane sulfur in biological samples.