Chemical Composition of Honeysuckle (Lonicerae japonicae) Extracts and Their Potential in Inhibiting the SARS-CoV-2 Spike Protein and ACE2 Binding, Suppressing ACE2, and Scavenging Radicals
Chemical Composition of Honeysuckle (Lonicerae japonicae) Extracts and Their Potential in Inhibiting the SARS-CoV-2 Spike Protein and ACE2 Binding, Suppressing ACE2, and Scavenging Radicals
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DOI：
10.1021/acs.jafc.3c00584
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发表时间：
2023-04-06
影响因子：
6.1
通讯作者：
Yu, Liangli Lucy
中科院分区：
文献类型：
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作者：
Gao, Boyan;Zhu, Lin;Yu, Liangli Lucy
蜂蜜已被用于功能性茶产品中。本研究对金银花水提物和醇提物的化学成分进行了研究，并沿着其抑制SARS-CoV-2刺突蛋白与ACE 2结合、抑制ACE 2活性和清除活性自由基的作用。采用HPLC-MS/MS技术从金银花提取物中初步鉴定出36个化合物，其中10个化合物为首次报道。两种金银花提取物均能抑制SARS-CoV-2刺突蛋白与ACE 2的结合，并抑制ACE 2的活性。乙醇提取物在100 mg植物当量/mL时对SARS-CoV-2刺突蛋白与ACE 2的结合表现出100%的抑制，而水提取物在相同浓度下具有65%的结合抑制。水提物对ACE 2活性的抑制率为90%，明显高于相同植物质量浓度下乙醇提取物的抑制率（62%）。此外，更高的总酚含量和更大的清除活性对羟基（HO中心点），DPPH中心点，ABTS中心点+自由基的水提取物中观察到比乙醇提取物对应的干植物重量浓度的基础上。这些发现表明金银花有潜力降低SARS-CoV-2感染和严重COVID-19症状发展的风险。
Honeysuckle (Lonicerae japonicae) has been used in functional tea products. The chemical compositions of the water and ethanol extracts of honeysuckle were examined in the present study, along with their potential in inhibiting SARS-CoV-2 spike protein binding to ACE2, suppressing ACE2 activity, and scavenging reactive free radicals. Thirty-six compounds were tentatively identified from the honeysuckle extracts using HPLC-MS/MS, with ten reported for the first time in honeysuckle. Both honeysuckle extracts inhibited the binding of SARS-CoV-2 spike protein to ACE2, as well as ACE2 activity. The ethanol extract exhibited a 100% inhibition on binding of the SARS-CoV-2 spike protein to ACE2 at 100 mg botanical equivalent/mL, whereas the water extract had a 65% binding inhibition at the same concentration. Furthermore, the water extract exhibited 90% ACE2 activity inhibition, which was stronger than that of the ethanol extract (62% inhibition) at the same botanical weight concentration. In addition, higher total phenolic contents and greater scavenging activities against hydroxyl (HO center dot), DPPH center dot, and ABTS center dot+ radicals were observed in the water extract than the ethanol extract counterpart on a dry botanical weight concentration basis. These findings suggest honeysuckle has the potential to reduce the risk of SARS-CoV-2 infection and the development of severe COVID-19 symptoms.