Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose
Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose
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DOI：
10.1128/aem.66.9.4058-4067.2000
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发表时间：
2000-09-01
影响因子：
4.4
通讯作者：
Tiedje, JM
中科院分区：
文献类型：
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作者：
Fernandez, AS;Hashsham, SA;Tiedje, JM
以产甲烷生物反应器群落作为模式生态系统，研究了功能稳定性与群落结构的关系。建立了具有两种不同群落结构的重复产甲烷生物反应器群落。通过形态学分析、小亚基（SSU）rRNA寡核苷酸探针、扩增核糖体DNA（rDNA）限制性分析（ARDIOR）和SSU rDNA克隆的部分测序，研究了基质负荷冲击对每个微生物群落种群动态的影响。一组复制的社区，指定的高螺旋体（HS）集，其特点是良好的可复制性，高比例的螺旋形和短细杆形态型，螺旋体相关的SSU rDNA基因的优势，和高比例的甲烷双螺旋体相关的SSU rRNA。第二组社区，指定的低螺旋体（LS）集，其特征在于不完全复制，较高的形态多样性占主导地位的球菌，链球菌相关的和深分支的螺旋体相关的SSU rDNA基因的优势，和甲烷菌相关的SSU rRNA的百分比高。在HS社区，葡萄糖扰动引起了巨大的变化，发酵细菌的相对丰度，与螺旋体相关的ribotypes由真杆菌相关的ribotypes暂时位移，然后返回到扰动前的社区结构。LS社区的扰动较小，与链球菌相关的生物体仍然普遍存在葡萄糖休克后，虽然变化的相对丰度的次要成员进行了形态分析。一篇配套论文表明，更稳定的LS社区在功能上不如HS社区稳定（S。A. Hashsham，A. S.费尔南德斯，S，L. Dollhopf，F. B。Dazzo，R，F，Hickey，J，M. Tiedje和C，S. Criddle，Appl. Environ. Microbiol. 66：4050-4057，2000）。
Methanogenic bioreactor communities mere used as model ecosystems to evaluate the relationship between functional stability and community structure. Replicated methanogenic bioreactor communities with two different community structures were established. The effect of a substrate loading shock on population dynamics in each microbial community was examined by using morphological analysis, small-subunit (SSU) rRNA oligonucleotide probes, amplified ribosomal DNA (rDNA) restriction analysis (ARDRA), and partial sequencing of SSU rDNA clones. One set of replicated communities, designated the high-spirochete (HS) set, was characterized by good replicability, a high proportion of spiral and short thin rod morphotypes, a dominance of spirochete-related SSU rDNA genes, and a high percentage of Methanosarcina-related SSU rRNA. The second set of communities, designated the low-spirochete (LS) set, was characterized by incomplete replicability, higher morphotype diversity dominated by cocci, a predominance of Streptococcus-related and deeply branching Spirochaetales-related SSU rDNA genes, and a high percentage of Methanosaeta-related SSU rRNA. In the HS communities, glucose perturbation caused a dramatic shift in the relative abundance of fermentative bacteria, with temporary displacement of spirochete-related ribotypes by Eubacterium-related ribotypes, followed by a return to the preperturbation community structure. The LS communities were less perturbed, with Streptococcus-related organisms remaining prevalent after the glucose shock, although changes in the relative abundance of minor members were detected by morphotype analysis. A companion paper demonstrates that the more stable LS communities were less functionally stable than the HS communities (S. A. Hashsham, A. S. Fernandez, S, L. Dollhopf, F. B. Dazzo, R, F, Hickey, J, M. Tiedje, and C, S. Criddle, Appl. Environ. Microbiol. 66:4050-4057, 2000).