Ontogenetic characterization of sporangium and spore of Huperzia serrata: an anti-aging disease fern
Ontogenetic characterization of sporangium and spore of Huperzia serrata: an anti-aging disease fern
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DOI：
10.1186/s40529-016-0151-9
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发表时间：
2016-11-14
影响因子：
3.4
通讯作者：
Li, Li
中科院分区：
文献类型：
--
作者：
Long, Hua;Li, Jing;Li, Li
背景：蛇足石杉是一种中药药用植物，已用于预防衰老疾病。它主要通过孢子繁殖，生长极其缓慢。由于严重捕捞，它是高度濒危物种。在本报告中，我们描述了与繁殖相关的孢子囊和孢子的个体发生。 H. serrata 植物的野生种群位于中国湖南省西部，并受到中国政府的保护，以研究其发育（例如孢子囊和孢子）和生态。田间观察和显微镜观察均进行了数年。结果：孢子囊从萌芽到成熟历时近1年。显微镜观察表明，孢子壁是由表皮细胞通过起始、细胞分裂和成熟发育而成。成熟孢子囊壁的结构由一层表皮、两层中层细胞和一层绒毡层组成。因此，孢子囊为真孢子囊类型。孢子发育分为表皮细胞起始、产孢细胞形成、初生产孢细胞、次生产孢细胞、孢子母细胞、四分体、成熟等6个阶段。结论：锯缘孢子囊发育属于真孢囊型。开发从启动到成熟大约需要1年时间。这些数据对于未来改善这种药用植物的繁殖很有用。
Background: Huperzia serrata is a medicinal plant used in Traditional Chinese Medicine, which has been used to prevent against aging diseases. It is mainly propagated by spores and grows extremely slowly. Due to severe harvest, it is a highly endangered species. In this report, we characterize ontogenesis of sporangia and spores that are associated with propagation. A wild population of H. serrata plants is localized in western Hunan province, China and protected by Chinese Government to study its development (e.g. sporangia and spores) and ecology. Both field and microscopic observations were conducted for a few of years.Results: The development of sporangia from their initiation to maturation took nearly 1 year. Microscopic observations showed that the sporangial walls were developed from epidermal cells via initiation, cell division, and maturation. The structure of the mature sporangial wall is composed of one layer of epidermis, two middle layers of cells, and one layer of tapetum. Therefore, the sporangium is the eusporangium type. Spore development is characterized into six stages, initiation from epidermal cell and formation of sporogenous cells, primary sporogenous cell, secondary sporogenous cell, spore mother cell, tetrad, and maturation.Conclusion: The sporangial development of H. serrata belongs to the eusporangium type. The development takes approximately 1 year period from the initiation to the maturation. These data are useful for improving propagation of this medicinal plant in the future.