BLOCKADE OF RECRUITMENT OF OVARIAN FOLLICLES BY SUPPRESSION OF THE SECONDARY SURGE OF FOLLICLE-STIMULATING-HORMONE WITH PORCINE FOLLICULAR-FLUID
BLOCKADE OF RECRUITMENT OF OVARIAN FOLLICLES BY SUPPRESSION OF THE SECONDARY SURGE OF FOLLICLE-STIMULATING-HORMONE WITH PORCINE FOLLICULAR-FLUID
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DOI：
10.1073/pnas.77.8.4953
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
SCHWARTZ, NB
中科院分区：
文献类型：
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作者：
HOAK, DC;SCHWARTZ, NB
在大鼠动情前期和动情期之间，卵泡刺激素(FSH；Follitropin)的血清浓度升高被认为是为了在下一个周期招募预定排卵的卵泡。发情前期晚期注射猪卵泡液(PFF)可抑制二次FSH峰；发情前期早期注射可抑制一次FSH峰而不影响二次FSH峰。可以使用PFF来检验FSH/卵泡募集假说，并区分原发和继发性FSH激增对这种募集的贡献。正常的卵泡募集发生在发情前期和发情期之间，直径在350-499微米之间。在发情前期晚期注射PFF抑制次级FSH峰时，在发情当天早晨注射PFF，但不注射猪血清(PS)，可阻止350-499微米大小组的卵泡募集。经PFF和PS处理的动物排卵数无差异。在发情前期早期注射PFF，仅抑制初级FSH峰时，PFF组和PS组动物在排卵数、卵泡大小分布和激素水平上无明显差异。在上一次实验中，次级FSH峰被PFF阻断，但被外源性绵羊FSH取代，导致卵泡募集的剂量相关增加，证实了卵泡液通过抑制FSH分泌来抑制募集的解释。在生殖周期短的哺乳动物中，促性腺激素激增提供了一种故障保护机制，促黄体生成素触发[促黄体生成素]排卵，结束一个周期，然后FSH水平的二次增加为下一个周期招募卵泡。
The increased serum concentration of follicle-stimulating hormone (FSH; follitropin) between proestrus and estrus in the rat was hypothesized to recruit the follicles destined to ovulate in the next cycle. Injection of porcine follicular fluid (PFF) late in proestrus suppresses the secondary FSH surge; injection early in proestrus suppresses the primary FSH surge without affecting the secondary FSH surge. It is possible to use PFF to test the FSH/follicular recruitment hypothesis and to distinguish between the contributions of the primary and secondary FSH surges to this recruitment. The normal recruitment of follicles occurs in the diameter range 350-499 .mu.m between the day of proestrus and the day of estrus. When the secondary FSH surge was suppressed by injection of PFF late in proestrus, PFF, but not porcine serum (PS), blocked follicular recruitment into size groups of 350-499 .mu.m on the morning of estrus. The number of ova ovulated did not differ between PFF- and PS-treated animals. When only the primary FSH surge was suppressed, by injecting PFF early in proestrus, there were no differences between PFF- and PS-treated animals in the number of ova ovulated, follicle size distribution or hormones. In the last experiment, the secondary FSH surge was blocked with PFF but was replaced with exogenous ovine FSH which caused a dose-related increase in follicular recruitment, substantiating the interpretation that the follicular fluid suppressed recruitment by suppressing FSH secretion. In mammals with short reproductive cycles, the gonadotropin surges provide a fail-safe mechanism whereby luteinizing hormone triggers [lutropin] ovulation, ending 1 cycle, and the secondary increase in FSH levels recruits follicles for the next cycle.