SELECTIVE ISOLATION OF ACETOBACTERIUM-WOODII ON METHOXYLATED AROMATIC-ACIDS AND DETERMINATION OF GROWTH YIELDS
SELECTIVE ISOLATION OF ACETOBACTERIUM-WOODII ON METHOXYLATED AROMATIC-ACIDS AND DETERMINATION OF GROWTH YIELDS
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DOI：
10.1007/bf00459530
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发表时间：
1981-01-01
影响因子：
2.8
通讯作者：
PFENNIG, N
中科院分区：
文献类型：
--
作者：
BACHE, R;PFENNIG, N
厌氧富集与甲氧基化芳香族化合物作为底物（香草酸，胡萝卜酸，三甲氧基肉桂酸）接种从淡水泥浆和污水污泥样品。在16个培养物中的12个中，选择性地富集了相同类型的杆状能动细菌。从纯培养中分离到两株菌株NZva 16和NZva 24，经鉴定为A. woodii与模式菌株DSM 1030比较。所有3种醋酸杆菌菌株都能够与所有10种测试的含有甲氧基的芳族化合物一起生长。在碳酸氢盐存在下，这些底物被用作唯一的有机电子供体和C源。紫外吸收光谱表明，芳环没有降解，并形成相应的羟基衍生物的甲氧基化化合物。形成的唯一进一步发酵产物是乙酸盐。当施加等摩尔浓度的甲氧基化苯甲酸衍生物时，生长产率与每个分子的甲氧基数目成比例。甲氧基或甲醇通过均乙酸发酵代谢：在碳酸氢盐存在下，4摩尔甲醇或甲氧基发酵成近3摩尔乙酸。在甲氧基化肉桂酸衍生物的情况下，形成较少的乙酸酯，并且由于丙烯酸酯侧链中的双键还原而出现相应的苯基丙酸的羟基衍生物。与具有相同数目甲氧基的苯甲酸酯衍生物相比，肉桂酸酯衍生物获得更高的生长产率。
Anaerobic enrichments with methoxylated aromatic compounds as substrates (vanillate, syringate, trimethoxycinnamate) were inoculated from freshwater mud and sewage sludge samples. In 12 of 16 cultures the same type of rod-shaped, motile bacteria was selectively enriched. Two strains, NZva 16 and NZva 24, were isolated in pure culture and recognized as A. woodii by comparison with the type strain (DSM 1030). All 3 Acetobacterium strains were able to grow with all 10 of the tested aromatic compounds containing methoxyl groups. In the presence of bicarbonate, these substrates were used as sole organic electron donors and C sources. UV-absorption spectra revealed that the aromatic rings were not degraded and that the corresponding hydroxy derivatives of the methoxylated compounds were formed. The only further fermentation product formed was acetate. When equimolar concentrations of the methoxylated benzoic acid derivatives were applied, the growth yields were proportional to the number of methoxyl groups per molecule. Methoxyl groups or methanol were metabolized by homoacetate fermentation: in the presence of bicarbonate 4 mol of methanol or methoxyl groups were fermented to nearly 3 mol of acetate. In case of the methoxylated cinnamic acid derivatives less acetate was formed and the corresponding hydroxy derivatives of phenylpropionic acid appeared as a result of the double bond reduction in the acrylate side chain. In comparison to the benzoate derivatives with the same number of methoxyl groups, higher growth yields were obtained with the cinnamate derivatives.