Polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties at each stage of fruit development in intersectional hybrids between highbush blueberry and shashanbo (Vaccinium bracteatum Thunb.)
Polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties at each stage of fruit development in intersectional hybrids between highbush blueberry and shashanbo (Vaccinium bracteatum Thunb.)
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DOI：
10.3233/jbr-210713
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发表时间：
2021-01-01
影响因子：
1.7
通讯作者：
Kunitake, Hisato
中科院分区：
文献类型：
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作者：
Toyama, Yuki;Toshima, Saki;Kunitake, Hisato
背景：在蓝莓中，蓝莓及其近缘果实的总多酚含量、总花青素含量和抗氧化活性正成为育种工作者的重要指标。最近，利用多倍体育种首次获得了日本野生种沙山伯与高丛蓝莓的杂交后代。目的：测定不同发育阶段的杂交果实的花色苷含量、多酚含量和抗氧化活性，探讨果实提取物对人早幼粒白血病(HL-60)细胞抗肿瘤细胞增殖特性的影响。方法：对3种杂交果实及其亲本在5个发育阶段的冻干果实进行多酚含量、花青素含量和两种抗氧化活性(DPPH和ORAC)的测定。以HL-60细胞存活率为指标，评价各提取物的抗肿瘤细胞增殖特性。结果：随着果实生长发育，果实中总多酚含量逐渐降低，成熟时含量略有增加。成熟期总花色苷含量增加幅度较大。此外，交叉杂交JM1的果实提取物在体外对HL-60人白血病细胞的生长有更强的抑制作用。总酚含量与抗氧化活性和抗肿瘤细胞增殖特性密切相关。结论：所制备的杂交种具有丰富的生物活性物质，具有较高的抗氧化活性和抗肿瘤细胞增殖特性。这一结果证明了利用日本野生越桔属植物进行蓝莓高活性化合物战略育种的有效性。
BACKGROUND: In blueberries, the total polyphenol contents, total anthocyanin contents, and antioxidant activities of fruits in blueberries and their relatives are becoming important targets for breeders. Recently, intersectional hybrids between Japanese wild species Shashanbo and highbush blueberry were produced using polyploid breeding for the first time. However, the polyphenol contents, antioxidant activities, and anti-cancer cell proliferation properties of the fruits of these intersectional hybrids have not been studied sufficiently.OBJECTIVE: The objectives of this study were to determine the anthocyanin contents, polyphenol contents, and antioxidant activities at each stage of fruit development in intersectional hybrids, and to examine the effects of the fruit extracts on anti-cancer cell proliferation properties using human promyelocytic leukemia (HL-60) cells.METHODS: Freeze-dried fruit samples of three intersectional hybrids and their parents at five developmental stages were used to evaluate their polyphenol contents, anthocyanin contents, and levels of two types of antioxidant activities (DPPH and ORAC). The anti-cancer cell proliferation properties of each extract were also evaluated according to the survival rate of HL-60 cells.RESULTS: The total polyphenol contents in fruits of the intersectional hybrids gradually decreased as the fruits grew, but the contents increased slightly at the mature stage. Meanwhile, the total anthocyanin contents increased strongly to the mature stage. In addition, the fruit extract of intersectional hybrid JM1 was found to be more effective for inhibiting the growth of HL-60 human leukemia cells in vitro. Total polyphenol content correlated highly with antioxidant activities and anti-cancer cell proliferation properties.CONCLUSIONS: We clarified that the intersectional hybrids had produced have abundant bioactive compounds, high levels of antioxidant activities, and anti-cancer cell proliferation properties. The results proved the effectiveness of the use of Japanese wild Vaccinium species for the strategic breeding of blueberries with high levels of bioactive compounds.