Wavelength dependence of cell cloning efficiency after optical trapping
Wavelength dependence of cell cloning efficiency after optical trapping
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DOI：
10.1016/s0006-3495(96)79716-3
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发表时间：
1996-03-01
影响因子：
3.4
通讯作者：
Berns, MW
中科院分区：
文献类型：
--
作者：
Liang, H;Vu, KT;Berns, MW
用Nd：YAG（1064 nm）和可调谐钛蓝宝石（700-990 nm）激光微束光阱对中国仓鼠卵巢（CHO）细胞进行了光捕获实验。将细胞核暴露于不同波长、功率密度和暴露持续时间的光捕获力。克隆生长一般随着功率密度和激光照射时间的增加而下降。观察到克隆生长的波长依赖性，在950-990 nm处具有最大克隆能力，在740-760 nm和900 nm处具有最小克隆能力。此外，最常用的捕获波长，1064 nm的Nd：YAG激光，强烈降低克隆能力，这取决于功率密度和曝光时间。本研究表明，各种光学参数时，必须考虑应用光学陷阱的生物问题的研究，特别是当生存和生存能力是重要因素。在解释实验结果时，必须认真考虑光阱改变用捕获光束探测的过程的结构或生物化学的能力。
A study on clonal growth in Chinese hamster ovary (CHO) cells was conducted after exposure to optical trapping wavelengths using Nd:YAG (1064 nm) and tunable titanium-sapphire (700-990 nm) laser microbeam optical traps. The nuclei of cells were exposed to optical trapping forces at various wavelengths, power densities, and durations of exposure. Clonal growth generally decreased as the power density and the duration of laser exposure increased. A wavelength dependence of clonal growth was observed, with maximum clonability at 950-990 nm and least clonability at 740-760 nm and 900 nm. Moreover, the most commonly used trapping wavelength, 1064 nm from the Nd:YAG laser, strongly reduced clonability, depending upon the power density and exposure time. The present study demonstrates that a variety of optical parameters must be considered when applying optical traps to the study of biological problems, especially when survival and viability are important factors. The ability of the optical trap to alter either the structure or biochemistry of the process being probed with the trapping beam must be seriously considered when interpreting experimental results.