Alterations of CCR5 and CCR7 Expression on Donor Peripheral Blood T Cell Subsets after Mobilization with rhG-CSF Correlate with Acute Graft-versus-host Disease
Alterations of CCR5 and CCR7 Expression on Donor Peripheral Blood T Cell Subsets after Mobilization with rhG-CSF Correlate with Acute Graft-versus-host Disease
复制标题
rhG-CSF 动员后供体外周血 T 细胞亚群 CCR5 和 CCR7 表达的变化与急性移植物抗宿主病相关
DOI：
10.1016/j.clim.2017.08.001
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Yu-Jun Dong
中科院分区：
文献类型：
--
作者：
Meng Wang;Jian Hu;Zhi-Xiang Qiu;Wei Liu;Mang-Ju Wang;Yuan Li;Yu-Hua Sun;Sai-Nan Zhu;Han-Yun Ren;Yu-Jun Dong
为研究重组人粒细胞集落刺激因子(rhG-CSF)对趋化因子受体的影响，探讨其诱导免疫耐受的可能机制，对97例家族性异基因造血干细胞移植供受者进行了研究。结果表明，动员后不同供者的基因表达变化有很大差异。多因素分析显示，动员后供者外周血中CD4+T细胞表达下调和人类白细胞抗原不匹配是急性移植物抗宿主病(GVHD)的独立危险因素。相反，CCR5对CD4+T细胞的下调与急性移植物抗宿主病的发生率降低有关。综上所述，rhG-CSF动员可导致不同供者T细胞亚群趋化因子受体表达的差异调节。供者CD4+T细胞CCR5表达下调和CCR7表达上调可能对急性GVHD有保护作用。这一发现可能为急性移植物抗宿主病的预防和治疗提供一种有希望的新策略。
To investigate the effects of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on chemokine receptors and explore the potential mechanism of rhG-CSF inducing immune tolerance, ninety-seven donor and recipient pairs undergoing family-donor allogeneic hematopoietic stem cell transplantation were studied. The results indicated that different donors showed great disparities in expression changes after mobilization. Multivariate analysis revealed that both HLA mismatching and CCR7 downregulation on donors' CD4+ T cells after mobilization were independent risk factors for acute graft-versus-host disease (GVHD). In contrast, CCR5 downregulation on CD4+ T cells was associated with reduced incidence of acute GVHD. In conclusion, rhG-CSF mobilization could lead to differential regulation of chemokine receptors expression on T cell subsets in different donors. Downregulation of CCR5 and upregulation of CCR7 expression on donor CD4+ T cells might protect recipients from acute GVHD. This finding may provide a promising new strategy for the prevention and treatment of acute GVHD.