Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg)
Application of sperm cryopreservation in selective breeding of the Pacific oyster, Crassostrea gigas (Thunberg)
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DOI：
10.1111/j.1365-2109.2008.02013.x
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发表时间：
2008-09-22
影响因子：
2
通讯作者：
Webb, Stephen C.
中科院分区：
文献类型：
--
作者：
Adams, Serean L.;Smith, John F.;Webb, Stephen C.
研究了太平洋牡蛎（Crassostreagigas（Thunberg））精子冷冻保存在家系选育中的稳健性。无论卵子密度如何，当使用1000：1至10 000：1的精卵比时，冷冻保存的精子均获得了较高的受精成功率。相同牡蛎批次的重复运行之间的变化是最小的，表明冷冻保存和幼虫饲养程序是可重复的。进行了20个独立的单雄-雌杂交，以评估冷冻保存的精子在选择性育种中的效用。未冷冻精子的生育力通常是冷冻精子生育力的不良预测因素。根据D-幼虫产量，20个杂交组合中有17个可能产生足够的鱼苗用于选择性育种（100万个卵中> 10（5）个D-幼虫），两个是边缘的（5 x 10（4）个D-幼虫），一个是不足的（4 x 10（3）个D-幼虫）。然后测试了一种替代受精策略，以提高给定数量精子的D产量。在精子：卵子比例为200：1的情况下，使1000万个卵子受精增加了总D-产量，而在精子：卵子比例为2000：1的情况下，相同的雄性-雌性配对增加了1000万个卵子。我们的结论是，尽管生育能力的变化很大，冷冻保存的精子是有用的家庭生产。
The robustness of Pacific oyster, Crassostrea gigas (Thunberg), sperm cryopreservation in the context of selective breeding based on family lines was investigated. Irrespective of egg density, high fertilization success was achieved with cryopreserved sperm when sperm:egg ratios of 1000:1 to 10 000:1 were used. Variation among replicate runs on the same oyster batches was minimal, indicating that cryopreservation and larval rearing procedures were repeatable. Twenty independent single male-female crosses were made to assess the utility of cryopreserved sperm in selective breeding. The fertility of unfrozen sperm was generally a poor predictor of cryopreserved sperm fertility. Based on D-larval yields, 17 of the 20 crosses were likely to yield adequate spat for selective breeding (> 10(5) D-larvae from 1 million eggs), two were marginal (5 x 10(4) D-larvae) and one was inadequate (4 x 10(3) D-larvae). An alternative fertilization strategy to improve D-yield from a given number of sperm was then tested. Fertilizing 10 million eggs at a sperm:egg ratio of 200:1 increased the total D-yield when compared with fertilizing 1 million eggs at a sperm:egg ratio of 2000:1 for the same male-female pair. We conclude that, despite wide variation in fertility, cryopreserved sperm is useful for family production.