The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X.
The synthesis of sulfated dextran beads for isolation of human plasma coagulation factors II, IX, and X.
复制标题
用于分离人血浆凝血因子 II、IX 和 X 的硫酸化葡聚糖珠的合成。
DOI：
10.1016/0003-2697(80)90462-5
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发表时间：
1980
影响因子：
2.9
通讯作者：
Majerus,PW
中科院分区：
文献类型：
--
作者：
Miletich,JP;BrozeJr,GJ;Majerus,PW
我们用球状聚合葡聚糖(Sephadex)与氯磺酸进行无水反应制备了一种新的凝血因子层析基质。该材料是分离人血浆凝血因子II、IX和X的有效吸附剂。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法均一的蛋白质回收率良好(II、IX和X因子分别为70%、40%和50%)，2天内可获得~gt；99%的无污染活性。这种新的层析材料大大简化了这些凝血因子的分离。
We have prepared a new matrix for the chromatography of coagulation factors using bead-polymerized dextran (Sephadex) sulfated by anhydrous reaction with chlorosulfonic acid. The material is a useful adsorbent for isolation of human plasma coagulation Factors II, IX, and X. Good recoveries (70, 40, and 50% for Factors II, IX, and X, respectively) of proteins homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and >99% free of contaminating activities can be obtained from human plasma in 2 days. This new chromatographic material greatly simplifies the isolation of these coagulation factors.