A physical map of the Escherichia coli K12 genome.
A physical map of the Escherichia coli K12 genome.
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大肠杆菌 K12 基因组的物理图谱。
DOI：
10.1126/science.3296194
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Cantor,CR
中科院分区：
文献类型：
--
作者：
Smith,CL;Econome,JG;Schutt,A;Klco,S;Cantor,CR
基因组的物理图谱就是它的DNA结构。构建这样的图谱是完整描述DNA特征的第一步。限制性内切酶Not I将大肠杆菌K12的基因组切成22个DNA片段，大小从20千碱基(20000碱基)到1000千碱基不等。这些都可以用脉冲场凝胶电泳法进行分离。根据AvailableE确定基因组中片段的顺序。共生遗传信息和部分消化模式的分析。得到的有序片段集是一个宏观限制图。这张地图为遗传和分子研究提供了便利。它的构建为进一步研究更大的基因组提供了一个模型。
A physical map of a genome is the structure of its DNA. Construction of such a map is a first step in the complete characterization of that DNA. The restriction endonuclease Not I cuts the genome ofEscherichia coliK12 into 22 DNA fragments ranging from 20 kilobases (20,000 base pairs) to 1000 kilobases. These can be separated by pulsed field gel electrophoresis. The order of the fragments in the genome was determined from availableE. coligenetic information and analysis of partial digest patterns. The resulting ordered set of fragments is a macrorestriction map. This map facilitates genetic and molecular studies onE. coli, and its construction serves as a model for further endeavors on larger genomes.