Molecular coordination of Staphylococcus aureus cell division.
Molecular coordination of Staphylococcus aureus cell division.
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DOI：
10.7554/elife.32057
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发表时间：
2018-02-21
期刊：
影响因子：
7.7
通讯作者：
Foster SJ
中科院分区：
文献类型：
--
作者：
Lund VA;Wacnik K;Turner RD;Cotterell BE;Walther CG;Fenn SJ;Grein F;Wollman AJ;Leake MC;Olivier N;Cadby A;Mesnage S;Jones S;Foster SJ
细菌细胞壁对于生存力是必不可少的，但是尽管其能够承受内部膨压，但必须保持动态以允许生长和分裂。肽聚糖是细胞壁的主要结构聚合物，其合成需要多种相互作用的组分。人类病原体金黄色葡萄球菌是在三个正交平面中分裂的长球体。在这里，我们已经整合了细胞形态分裂与分子水平分辨率成像肽聚糖合成和负责的组件。合成发生在整个发展中的间隔表面的扩散模式，所观察到的间隔几何形状的必要性，这是匹配的杂色分裂成分分布。合成在隔环完成后继续，其中核心分裂组件FtsZ仍然存在。新的分子水平的信息需要重新评估的生长和分裂过程，导致一个新的概念模型，从而加快了细胞周期的一组功能连接，但不规则分布的组件。
The bacterial cell wall is essential for viability, but despite its ability to withstand internal turgor must remain dynamic to permit growth and division. Peptidoglycan is the major cell wall structural polymer, whose synthesis requires multiple interacting components. The human pathogen Staphylococcus aureus is a prolate spheroid that divides in three orthogonal planes. Here, we have integrated cellular morphology during division with molecular level resolution imaging of peptidoglycan synthesis and the components responsible. Synthesis occurs across the developing septal surface in a diffuse pattern, a necessity of the observed septal geometry, that is matched by variegated division component distribution. Synthesis continues after septal annulus completion, where the core division component FtsZ remains. The novel molecular level information requires re-evaluation of the growth and division processes leading to a new conceptual model, whereby the cell cycle is expedited by a set of functionally connected but not regularly distributed components.