DNA repair and the evolution of transformation in Bacillus subtilis. III. Sex with damaged DNA.
DNA repair and the evolution of transformation in Bacillus subtilis. III. Sex with damaged DNA.
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DNA 修复和枯草芽孢杆菌转化的进化。
DOI：
10.1093/genetics/128.2.215
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发表时间：
1991
期刊：
影响因子：
3.3
通讯作者：
Michod,RE
中科院分区：
文献类型：
--
作者：
Hoelzer,MA;Michod,RE
枯草芽孢杆菌的自然遗传转化为研究有性重组的进化功能提供了一个实验系统。修复假说提出，在转化过程中，细胞摄取的外源DNA被用作重组修复受体细胞基因组中损伤的模板。早期的结果表明，转化细胞的群体密度（即，与未转化的细胞（主要是无性细胞）相比，如果细胞在受到损伤后用未损伤的同源DNA转化，则随着紫外线照射剂量的增加，转化的细胞（主要是有性细胞）的DNA含量增加。然而，在自然界中，用于转化的供体DNA可能来自与受体细胞一样受损的细胞。为了更好地模拟自然群体中转化的效果，我们使用受损的供体DNA进行了与刚才描述的那些类似的实验。我们在本报告中记录了转化体的相对密度继续增加，即使它们用受损的供体DNA转化。这些结果表明，转化位点通常是受体细胞基因组中受损的位点。
Natural genetic transformation in the bacterium Bacillus subtilis provides an experimental system for studying the evolutionary function of sexual recombination. The repair hypothesis proposes that during transformation the exogenous DNA taken up by cells is used as template for recombinational repair of damages in the recipient cell's genome. Earlier results demonstrated that the population density of transformed cells (i.e., sexual cells) increases, relative to nontransformed cells (primarily asexual cells), with increasing dosage of ultraviolet irradiation, provided that the cells are transformed with undamaged homologous DNA after they have become damaged. In nature, however, donor DNA for transformation is likely to come from cells that are as damaged as the recipient cells. In order to better simulate the effects of transformation in natural populations we conducted similar experiments as those just described using damaged donor DNA. We document in this report that transformants continue to increase in relative density even if they are transformed with damaged donor DNA. These results suggest that sites of transformation are often damaged sites in the recipient cell's genome.