Proton NMR spectroscopy of solvent-saturable resonances:: A new approach to study pH effects in situ
Proton NMR spectroscopy of solvent-saturable resonances:: A new approach to study pH effects in situ
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DOI：
10.1002/mrm.1910400105
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发表时间：
1998-07-01
影响因子：
3.3
通讯作者：
van Zijl, PCM
中科院分区：
文献类型：
--
作者：
Mori, S;Eleff, SM;van Zijl, PCM
结果表明，可以通过与水交换的代谢物质子的信号强度的pH敏感性在质子NMR光谱中测量pH变化的作用。为了观察这种现象，他必须使用脉冲序列，才能在生理条件下敏感地观察这些可交换的质子，这是通过避免由于水饱和而避免使用磁化剂转移信号套件来实现的，以实现溶剂抑制的目的。这些方法提供了5至10 ppm之间许多信号的缩放级增强，其中包含n键质子以及与它们耦合的芳族C-H质子，其强度受到交换延迟饱和的影响。作为第一个应用，研究了pH变化对这些共振的影响（灌注细胞）和体内（CAT脑）。
It is shown that the effect of pH changes can be measured in proton NMR spectra through the pH sensitivity of the signal intensities of metabolite protons exchanging with water. To observe this phenomenon, pulse sequences must he used that can sensitively observe these exchangeable protons under physiological conditions, which is achieved by avoiding magnetization transfer signal lasses due to water saturation for solvent suppression purposes. These methods provide an order-of-magnitude enhancement of many signals between 5 and 10 ppm, containing both N-bound protons as well as aromatic C-H protons coupled to them, the intensity of which is influenced by exchange-relayed saturation. As a first application, the effects of pH change on these resonances are studied ex vivo (perfused cells) and in vivo (cat brain).