LeERF1 positively modulated ethylene triple response on etiolated seedling, plant development and fruit ripening and softening in tomato
LeERF1 positively modulated ethylene triple response on etiolated seedling, plant development and fruit ripening and softening in tomato
复制标题
LeERF1正向调节番茄黄化幼苗、植物发育以及果实成熟和软化的乙烯三重反应
DOI：
10.1007/s00299-007-0394-8
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发表时间：
2007-11-01
影响因子：
6.2
通讯作者：
Luo, Yunbo
中科院分区：
文献类型：
--
作者：
Li, Yingcong;Zhu, Benzhong;Luo, Yunbo
为研究LeERF 1基因在乙烯三重反应中的作用，将LeERF 1基因导入番茄（Lycopersicon esculentum cv.中薯4号）的遗传转化。获得了正义LeERF 1和反义LeERF 1转基因番茄。过量表达LeERF 1可引起番茄黄化苗产生典型的乙烯三重反应。在成株期，35 S：：LeERF 1使LeERF 1-sn株系的叶片发生形态学变化。与野生型番茄相比，反义LeERF 1番茄果实的货架期延长。结果表明，LeERF 1正向介导乙烯信号，并以LeERF 1-sn、wt和LeERF 1-为材料，首次证实了LeERF 1在番茄黄化苗、植株发育和果实成熟软化过程中与乙烯三重反应呈正相关。
To study the function of LeERF1 in ethylene triple response on etiolated seedling, plant development and fruit ripening and softening, LeERF1 gene was introduced into tomato (Lycopersicon esculentum cv. No. 4 Zhongshu) through Agrobacterium-mediated transformation. The sense LeERF1 and anti-sense LeERF1 transgenic tomato were obtained. Overexpression of LeERF1 in tomato caused the typical ethylene triple response on etiolated seedling. In the adult stage, 35S::LeERF1 resulted in morphological changes in the leaves of the LeERF1-sn lines. Anti-sense LeERF1 fruits had longer shelf life compared with wild-type tomato. The results of this manuscript indicated that LeERF1 positively mediated the ethylene signals, while the function of LeERF1 was verified for the first time to be positively related with ethylene triple response on etiolated seedling, plant development and fruit ripening and softening using LeERF1-sn, wt and LeERF1-as tomato.