Determination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry.
Determination of testosterone in human hair by gas chromatography-selected ion monitoring mass spectrometry.
复制标题
采用气相色谱-选择离子监测质谱法测定人发中的睾酮。
DOI：
10.1039/a805131c
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
S. Wudy
中科院分区：
文献类型：
--
作者：
C. Scherer;U. Wachter;S. Wudy
建立了以d3-睾酮为内标物，用气相色谱-质谱法测定人发中睾酮的方法。方法采用碱消化、快速液-液萃取、LH-20柱层析、七氟丁酸酐衍生化。通过m/z 680（睾酮）和m/z 683（d3-睾酮）的选择离子监测实现定量。我们的方法不需要对同位素贡献进行复杂的校正。该方法提供了一种灵敏、特异的技术，具有良好的准确度和精密度。这是第一次，睾丸激素已被量化的气相色谱-质谱法在人类头发。浓度（中位数，范围，ng g-1毛发）反映了显著（p = 0.05; t检验）性别差异，男性毛发中为2.7 ng g-1（2.5-4.2），女性毛发中为1.7 ng g-1（1.0-3.4）。
A method for the determination of testosterone in human hair by gas chromatography-mass spectrometry using d3-testosterone as internal standard is described. Our method consisted of alkaline digestion, fast liquid-liquid extraction, LH-20 chromatography and derivatization with heptafluorobutyric anhydride. Quantification was achieved by selected ion monitoring of m/z 680 (testosterone) and m/z 683 (d3-testosterone). Our method needed no complex corrections for isotope contributions. The procedure provided a sensitive and specific technique with good accuracy and precision. For the first time, testosterone has been quantified by gas chromatography-mass spectrometry in human hair. The concentrations (median, range, ng g-1 hair) reflected a significant (p = 0.05; t-test) sex difference with 2.7 ng g-1 (2.5-4.2) in male and 1.7 ng g-1 (1.0-3.4) in female hair.
检测合成代谢类固醇给药：尿睾酮与表睾酮的比率与尿睾酮与黄体生成素的比率。
影响因子：
9.3
作者：
Perry,PJ;MacIndoe,JH;Yates,WR;Scott,SD;Holman,TL
通讯作者：
Holman,TL