Ultrasensitive sandwich RNA-aptasensor based on dual-signal amplification strategy for highly sensitive neomycin detection
Ultrasensitive sandwich RNA-aptasensor based on dual-signal amplification strategy for highly sensitive neomycin detection
复制标题
DOI：
10.1016/j.foodcont.2021.108445
复制
发表时间：
--
期刊：
影响因子：
6
通讯作者：
Falan Li;Xiaolin Gao;Xiaoyang Wang;Yemin Guo;Xia Sun;Qingqing Yang;Yanyan Zhang
中科院分区：
文献类型：
--
作者：
Falan Li;Xiaolin Gao;Xiaoyang Wang;Yemin Guo;Xia Sun;Qingqing Yang;Yanyan Zhang
本研究旨在设计一种基于双信号放大策略的高灵敏度电化学配体传感器，用于检测牛奶样品中的新霉素（NEO）。由于二茂铁（Fc）的存在，多壁碳纳米管（MWCNTs）的电子信号得到了极大的改善，其具有良好的电子传递能力。采用水热法和化学活化法制备了二氧化硅杂化介孔氧化铁纳米颗粒（Fe3O4@SiO2），该纳米颗粒具有较大的比孔容和比表面积，可以固定更多的RNA适体（Apts），促进纳米颗粒标记的电子转移。两个Apts （Apt1和Apt2）和靶标之间形成了一个复合三明治。此外，纳米金修饰Apt2增强了电化学信号。利用循环伏安法（CV）和差分脉冲伏安法（DPV）对该传感器的性能进行了详细表征。在优化条件下，电化学传感器的响应电流差与NEO浓度的正对数呈线性关系，在5 × 10−3nM ~ 5 × 102nM范围内，检出限为0.759 nM。电化学配体传感器具有特异性和选择性。此外，所构建的适配体传感器已成功应用于牛奶中NEO的检测。这些结果表明，该传感器在近地天体的实际监测中具有潜在的应用前景。
This study aimed to design a highly sensitive electrochemical aptasensor based on the dual-signal amplification strategy for detecting neomycin (NEO) in milk samples. The electronic signal of multi-walled carbon nanotubes (MWCNTs) was greatly improved due to the existence of ferrocene (Fc), which had good electron transfer ability. The silica hybridized mesoporous ferroferric oxide nanoparticles (Fe3O4@SiO2) were prepared by hydrothermal and chemical activation treatments, which had a large specific pore volume and a large specific surface area, they could fix more RNA aptamers (Apts) and promote the electron transfer of nanoparticle labels. A composite sandwich was formed between the two Apts (Apt1 and Apt2) and the target. Also, Apt2 was modified by gold nanoparticles (AuNPs), enhancing the electrochemical signal. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were used to characterize the performance of the aptasensor in detail. Under optimized conditions, the response current differences of the electrochemical aptasensor exhibited a linear relationship toward the positive logarithm of NEO concentrations ranging from 5 × 10−3nM to 5 × 102nM and a detection limit of 0.759 nM. The electrochemical aptasensor showed specificity and selectivity. In addition, the constructed aptasensor was successfully applied to the detection of NEO in milk. These results indicated that the aptasensor had potential applications in the actual monitoring of NEO.