CONTINUED HIGH ALBUMIN PRODUCTION BY MULTICELLULAR SPHEROIDS OF ADULT-RAT HEPATOCYTES FORMED IN THE PRESENCE OF LIVER-DERIVED PROTEOGLYCANS
CONTINUED HIGH ALBUMIN PRODUCTION BY MULTICELLULAR SPHEROIDS OF ADULT-RAT HEPATOCYTES FORMED IN THE PRESENCE OF LIVER-DERIVED PROTEOGLYCANS
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DOI：
10.1016/0006-291x(89)91609-4
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发表时间：
1989-05-30
影响因子：
3.1
通讯作者：
TSUJI, T
中科院分区：
文献类型：
--
作者：
KOIDE, N;SHINJI, T;TSUJI, T
当成年大鼠肝细胞与从大鼠肝脏网状蛋白纤维中分离出的蛋白聚糖组分一起培养时，形成漂浮的多细胞球体。球体中的细胞仅表现出低生长活性。在前 6 天内，球体的白蛋白产量增加至 1.5 μg/μg DNA/天（180 μg/mg 蛋白质/天），此后保持恒定。相反，4天后单层的白蛋白产量显着下降。球状培养物似乎比单层培养物更适合研究成体肝细胞的分化功能。
Adult rat hepatocytes formed floating multicellular spheroids, when they were cultured with proteoglycan fraction isolated from rat liver reticulin fibers. Cells in the spheroid showed only low growth activity. Albumin production by the spheroids increased up to 1.5 .mu.g/.mu.g DNA/day (180 .mu.g/mg protein/day) during the first 6 days and remained constant thereafter. In contrast, the albumin production by the monolayer markedly decreased after 4 days. The spheroid culture appears to be more suitable than the monolayer in studying differentiated functions of adult hepatocytes.