Aptitude of Auricular and Nasoseptal Chondrocytes Cultured Under a Monolayer or Three-Dimensional Condition for Cartilage Tissue Engineering
Aptitude of Auricular and Nasoseptal Chondrocytes Cultured Under a Monolayer or Three-Dimensional Condition for Cartilage Tissue Engineering
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DOI：
10.1089/ten.tea.2007.0218
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发表时间：
2009-05-01
影响因子：
4.1
通讯作者：
Hoshi, Kazuto
中科院分区：
文献类型：
--
作者：
Asawa, Yukiyo;Ogasawara, Toru;Hoshi, Kazuto
为了阐明源自耳廓软骨（供体：10-15岁）和鼻中隔软骨（20-23岁）的软骨细胞的特征，我们评估了单层和I型胶原（COL 1）三维（3D）条件下培养的两种细胞的增殖或基质合成。3次传代，直到耳廓软骨细胞在3D培养中的细胞数增加1000倍，尽管单层培养或鼻中隔单层和3D细胞在4次传代时达到1000倍的增加。当我们培养由1000倍增殖的软骨细胞制成的组织工程软骨颗粒时，单层细胞的颗粒在培养期间保持其大小。然而，鼻中隔3D细胞在第1天开始缩小，在第21天变得大约十分之一的大小。颗粒的缩小可能是由于肿瘤坏死因子（TNF）-α或相关蛋白酶（包括基质金属蛋白酶（MMP）-1、-2和-3以及组织蛋白酶B）的上调，这表明在生理上与COL 1分离的鼻中隔软骨细胞可能很难适应COL 1 3D增殖条件。理想情况下，这些特征将在来自年龄完全匹配的供体的软骨细胞之间进行比较。然而，根据我们的数据使用密切匹配的，耳廓软骨细胞似乎更迅速地增殖，并产生较少的蛋白酶在此3D文化比鼻中隔的。
To elucidate the characterizations of chondrocytes originating from auricular cartilage (donors: 10-15 years) and nasoseptal one (20-23 years), we evaluated proliferation or matrix synthesis of both cells cultured under monolayer and collagen type I (COL1) three-dimensional (3D) conditions. Three passages were needed until cell numbers of auricular chondrocytes in the 3D culture increased 1000-fold, although those in monolayer culture or nasoseptal monolayer and 3D cells reached a 1000-fold increase at four passages. When we cultured the tissue-engineered cartilage pellets made of the chondrocytes proliferated at 1000-fold increase, the pellets of monolayer cells maintained their sizes during the culture period. However, those of nasoseptal 3D cells began to shrink at day 1 and became approximately one-tenth in size at day 21. The downsizing of pellets may result from the upregulation of tumor necrosis factor (TNF)-alpha or the related proteinases, including matrix metalloproteinases (MMPs)-1, -2, and -3, and cathepsin B, suggesting that the nasoseptal chondrocytes, which are physiologically separated from COL1, may be hardly adapted for the COL1 3D proliferation condition. Ideally, these characteristics would have been compared between the chondrocytes from donors that are completely matched in ages. However, according to our data using closely matched ones, the auricular chondrocytes seemed to more rapidly proliferate and produce less proteinases during this 3D culture than the nasoseptal ones.