Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage galactose-type C-type lectin-1-deficient mouse embryos
Retardation of removal of radiation-induced apoptotic cells in developing neural tubes in macrophage galactose-type C-type lectin-1-deficient mouse embryos
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DOI：
10.1093/glycob/cwj028
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发表时间：
2005-12-01
期刊：
影响因子：
4.3
通讯作者：
Irimura, T
中科院分区：
文献类型：
--
作者：
Yuita, H;Tsuiji, M;Irimura, T
MGL1/CD301a 是一种 C 型凝集素，可将半乳糖和 N-乙酰半乳糖胺识别为单糖，并在有限的巨噬细胞和树突细胞群中表达，至少在成年小鼠中如此。在这项研究中，具有 Mgl1+/- 基因型的怀孕小鼠与 Mgl1+/- 或 Mgl1-/- 基因型雄性小鼠交配，胚胎用于评估该分子在凋亡细胞清除中发挥重要作用的假设。在性交后 10.5 天（d.p.c.）对发育中的胚胎进行 1 Gy 的 X 射线照射后，与 Mgl1+/+ 幼崽相比，Mgl1-/- 幼崽的数量显着减少。对受辐射的 Mgl1+/+ 胚胎中 MGL1 阳性细胞、MGL2 阳性细胞和凋亡细胞的分布进行组织学检查。在神经管中检测到MGL1阳性细胞，其中许多细胞发生凋亡，而没有观察到MGL2阳性细胞。生物素化的重组 MGL1 与大部分凋亡细胞结合。当检查 Mgl1+/+ 和 Mgl1-/- 胚胎是否存在凋亡细胞时，出现相似数量的凋亡细胞，但 Mgl1-/- 胚胎中这些细胞的清除速度慢于 Mgl1+/+ 胚胎。这些结果强烈表明 MGL1/CD301a 参与凋亡细胞的清除。这个过程对于 X 射线照射的胚胎的修复和正常发育至关重要。
MGL1/CD301a is a C-type lectin that recognizes galactose and N-acetylgalactosamine as monosaccharides and is expressed on limited populations of macrophages and dendritic cells at least in adult mice. In this study, pregnant mice with Mgl1+/- genotype were mated with Mgl1+/- or Mgl1-/- genotype males, and the embryos were used to assess a hypothesis that this molecule plays an important role in the clearance of apoptotic cells. After X-ray irradiation at 1 Gy of developing embryos at 10.5 days post coitus (d.p.c.), the number of Mgl1-/- pups was significantly reduced as compared with Mgl1+/+ pups. Distributions of MGL1-positive cells, MGL2-positive cells, and apoptotic cells were histologically examined in irradiated Mgl1+/+ embryos. MGL1-positive cells were detected in the neural tube in which many cells undergo apoptosis, whereas MGL2-positive cells were not observed. Biotinylated recombinant MGL1 bound a significant portion of the apoptotic cells. When Mgl1+/+ and Mgl1-/- embryos were examined for the presence of apoptotic cells, similar numbers of apoptotic cells gave rise, but the clearance of these cells was slower in Mgl1-/- embryos than in Mgl1+/+ embryos. These results strongly suggest that MGL1/CD301a is involved in the clearance of apoptotic cells. This process should be essential in the repair and normal development of X-ray-irradiated embryos.