Reduced expression of aquaporin 9 in tubal ectopic pregnancy
Reduced expression of aquaporin 9 in tubal ectopic pregnancy
复制标题
输卵管异位妊娠中水通道蛋白9表达降低
DOI：
10.1007/s10735-012-9471-6
复制
发表时间：
2013-04-01
影响因子：
3.2
通讯作者：
Shanguan, Xue Jun
中科院分区：
文献类型：
--
作者：
Ji, Yin Fen;Chen, Li You;Shanguan, Xue Jun
以往的研究表明，在蜕膜形成和胚胎着床过程中，被动水通道(AQPs)在维持子宫内膜细胞膜水分动态平衡方面具有重要作用。然而，有关水通道蛋白在人类输卵管中的作用，特别是在人类输卵管异位妊娠中的作用的信息很少。在本研究中，我们采集了输卵管妊娠植入部位(组1，N=2.30，平均年龄32岁，范围23~42岁)和输卵管切除术后相应的非植入部位(组2)的组织标本。取分泌期中期的壶腹输卵管作为对照组(第3组，N=417，平均年龄37岁，30~50岁)。制备组织切片，分别进行抗AQP9和雌孕激素受体染色。免疫组织化学研究表明，AQP9蛋白定位于输卵管上皮细胞的胞浆中。AQP9在输卵管妊娠中的表达明显低于对照组(第1组与第3组，P=0.0.036；第2组与第3组，P=0.029)，且这种表达降低与雌孕激素受体状态无关(第2组，ER与AQP9比较，P=0.0.173，P=0.361；PR与AQP9相比，P=0.0.124，P=0.514)。同样，正常组雌孕激素受体状态与AQP9无相关性(第3组，ER与AQP9，皮尔逊相关系数分别为−0.026，P=0.923；PR与AQP9，皮尔逊相关系数=−0.292，P=0.255)。AQP9在人输卵管中的表达减少可能参与了输卵管妊娠的病理生理过程。
Previous studies demonstrate significant roles for passive water channels (aquaporins, AQPs) in maintaining water homeostasis in cell membranes of endometrial cells during decidualisation and embryo implantation. However, there is little information regarding the role of AQPs in the human fallopian tube, specifically their role in human tubal ectopic pregnancy. In this study we took tissue samples from the site of implantation of tubal ectopic pregnancy (group 1, N = 30, mean age 32 years, range 23–42) and the corresponding non-implantation site in women undergoing salpingectomy for tubal pregnancy (group 2). Ampullary fallopian tubes during mid-secretory phase were collected as control group (group 3, N = 17, mean age 37 years, range 30–50). Thin sections were prepared and stained with anti-AQP9, and, for estrogen and progesterone receptors in each group. Immunohistochemical studies showed that AQP9 proteins localize in the cytoplasm of epithelial cells of Fallopian tube. Expression of AQP9 was significantly reduced during tubal pregnancy compared to controls (group 1 vs. group 3,P= 0.036; group 2 vs. group 3,P= 0.029), and, this reduced expression was not related to estrogen receptor or progesterone receptor status (group 2, ER vs. AQP9, Pearson r = 0.173,P= 0.361; PR vs. AQP9, Pearson r = 0.124,P= 0.514, respectively). Similarly, there is no correlation between AQP9 and estrogen receptor or progesterone receptor status in the normal group (group 3, ER vs. AQP9, Pearson r = −0.026,P= 0.923; PR vs. AQP9, Pearson r = −0.292,P= 0.255, respectively). Reduced expression of AQP9 in human fallopian tube may contribute to aspects of pathophysiology of tubal ectopic pregnancy.