Directing the osteoblastic and chondrocytic differentiations of mesenchymal stem cells: matrix vs. induction media
Directing the osteoblastic and chondrocytic differentiations of mesenchymal stem cells: matrix vs. induction media
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指导间充质干细胞的成骨细胞和软骨细胞分化：基质与诱导培养基
DOI：
10.1093/rb/rbx008
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发表时间：
2017-10-01
影响因子：
6.7
通讯作者：
Wu, Fang
中科院分区：
文献类型：
--
作者：
He, Jing;Guo, Jianglong;Wu, Fang
摘要虽然诱导培养基和基质都被报道可以调节干细胞的命运，但对于哪种因子在指导MSC分化谱系中起着更决定性的作用以及潜在的机制却知之甚少。为此，我们将MSC接种在HA-胶原和HA-合成水凝胶基质上，这两种基质分别表现出高度不同的成骨细胞和软骨细胞分化谱系的潜力，并分别用成骨、成软骨和正常培养基培养它们。系统比较了诱导培养基和基质对MSC粘附、细胞骨架组织、增殖，特别是分化成成骨细胞和软骨细胞谱系的影响。结果表明，基质选择比诱导培养基对定向分化谱系有更深刻的影响。在我们的研究中，经典的Wnt-β-Catenin信号通路直接参与了基质驱动的成骨细胞分化，这种对转录活性的强烈调节作用可能是导致上述观察结果的关键因素。这些发现不仅提供了一个关键的洞察自然细胞事件导致成骨细胞和软骨细胞分化，但也有重要的意义，在生物材料设计的组织工程应用。
Abstract While both induction culture media and matrix have been reported to regulate the stem cell fate, little is known about which factor plays a more decisive role in directing the MSC differentiation lineage as well as the underlying mechanisms. To this aim, we seeded MSCs on HA-collagen and HA-synthetic hydrogel matrixes, which had demonstrated highly different potentials toward osteoblastic and chondrocytic differentiation lineages, respectively, and cultured them with osteogenic, chondrogenic and normal culture media, respectively. A systematic comparison has been carried out on the effects of induction media and matrix on MSC adhesion, cytoskeleton organization, proliferation, and in particular differentiation into the osteoblastic and chondrocytic lineages. The results demonstrated that the matrix selection had a much more profound effect on directing the differentiation lineage than the induction media did. The strong modulation effect on the transcription activities might be the critical factor contributing to the above observations in our study, where canonical Wnt-β-Catenin signal pathway was directly involved in the matrix-driven osteoblastic differentiation. Such findings not only provide a critical insight on natural cellular events leading to the osteoblastic and chondrocytic differentiations, but also have important implications in biomaterial design for tissue engineering applications.