IL-2 and IFN-gamma are two necessary lymphokines in the development of cytolytic T cells.
IL-2 and IFN-gamma are two necessary lymphokines in the development of cytolytic T cells.
复制标题
DOI：
10.4049/jimmunol.143.4.1210
复制
发表时间：
1989-08
影响因子：
4.4
通讯作者：
E. Maraskovsky;W. F. Chen;K. Shortman
中科院分区：
文献类型：
--
作者：
E. Maraskovsky;W. F. Chen;K. Shortman
建立了一种无填充细胞的极限稀释微培养系统，用于扩增和分化高比例的单个CD4-CD8+T细胞为细胞溶解T细胞(CTL)克隆。刺激物为PMA和钙离子载体离子霉素。生长和分化因子为rIL-2，外加ConA刺激的脾细胞上清液(CAS)或rIFN-γ。用放射自显影111In释放法检测CTL活性。在CAS和rIL-2存在的情况下，50%的潜在前体(CTL-p)产生了溶细胞克隆。用重组干扰素-γ替代CAS的效率与高达42%的CTL-p产生细胞溶解克隆的效率相似。单独使用rIL-2只允许一小部分(6%)的CD4-CD8+T细胞成为溶细胞克隆。在培养的不同阶段加入rIL-2和r干扰素-γ表明，IL-2在整个培养过程中都是必需的，但只有在早期阶段才需要外源性的干扰素-γ。结论：在至少40%的CTL-p中，IL-2和干扰素-γ是必不可少的两种淋巴因子，它们协同作用诱导CTL增殖和分化。
A filler cell-free limiting-dilution microculture system has been developed for the expansion and differentiation of a high proportion of single CD4-CD8+ T cells into cytolytic T cell (CTL) clones. The stimulus used was PMA together with the calcium ionophore ionomycin. The growth and differentiation factors were rIL-2, together with either a Con A-stimulated spleen cell supernatant (CAS) or rIFN-gamma. CTL activity was monitored by an autoradiographic 111In-release assay. With CAS and rIL-2 present, 50% of all potential precursors (CTL-p) produced cytolytic clones. Substitution of rIFN-gamma for CAS gave a similar efficiency with up to 42% of CTL-p producing cytolytic clones. rIL-2 alone allowed only a small proportion (6%) of CD4-CD8+ T cells to become cytolytic clones. Addition of rIL-2 and rIFN-gamma at various stages of the culture demonstrated that IL-2 was required throughout, but exogenous IFN-gamma was required only during the early stages. It is concluded that for at least 40% of all CTL-p, the lymphokines IL-2 and IFN-gamma are essential and act in synergy to induce proliferation and differentiation into CTL.