Homologs of small nucleolar RNAs in archaea
Homologs of small nucleolar RNAs in archaea
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DOI：
10.1126/science.288.5465.517
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发表时间：
2000-04-21
期刊：
影响因子：
56.9
通讯作者：
Dennis, PP
中科院分区：
文献类型：
--
作者：
Omer, AD;Lowe, TM;Dennis, PP
在真核生物中，数十种转录后修饰通过小核仁RNA（snoRNA）针对核糖体RNA（rRNA）中的特定核苷酸。我们确定了同源的snoRNA基因在这两个分支中的的的的spacea。通过与真核snoRNA相关蛋白的同源物古细菌原纤蛋白和NOP 56的免疫共沉淀，从古细菌酸热硫化叶菌中克隆了18个小sno样RNA（sRNA）。我们在这些sRNA上训练了一个概率模型，以在古细菌基因组序列中搜索更多的sRNA。在代表Crenarchaeota和Euryarchaeota的7个古细菌基因组中鉴定出超过200个额外的sRNA，因此基于snoRNA的rRNA加工可能存在于古细菌和真核生物最后的共同祖先中，早于形态上不同的核仁的进化。
In eukaryotes, dozens of posttranscriptional modifications are directed to specific nucleotides in ribosomal RNAs (rRNAs) by small nucleolar RNAs (snoRNAs). We identified homologs of snoRNA genes in both branches of the Archaea. Eighteen small sno-like RNAs (sRNAs) were cloned from the archaeon Sulfolobus acidocaldarius by coimmunoprecipitation with archaeal fibrillarin and NOP56, the homologs of eukaryotic snoRNA-associated proteins. We trained a probabilistic model on these sRNAs to search for more sRNAs in archaeal genomic sequences. Over 200 additional sRNAs were identified in seven archaeal genomes representing both the Crenarchaeota and the Euryarchaeota, snoRNA-based rRNA processing was therefore probably present in, the Last common ancestor of Archaea and Eukarya, predating the evolution of a morphologically distinct nucleolus.