Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite ceramic
Low-intensity pulsed ultrasound increases bone ingrowth into porous hydroxyapatite ceramic
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DOI：
10.1007/s00774-007-0777-5
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发表时间：
2007-11-01
影响因子：
3.3
通讯作者：
Tsumaki, Noriyuki
中科院分区：
文献类型：
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作者：
Iwai, Takao;Harada, Yoshifumi;Tsumaki, Noriyuki
由羟基磷灰石（HA）制成的合成多孔陶瓷已被用作骨移植替代物。在本研究中，我们研究了低强度脉冲超声（LIPUS）是否加速骨长入HA陶瓷的孔隙。应用LIPUS不会机械削弱浸在水中的多孔陶瓷。使用家兔的体内实验表明，与未暴露于LIPUS的类似植入HA陶瓷相比，LIPUS应用2周显著增加了植入股骨髁的多孔HA陶瓷中心部分的成骨细胞数量和骨面积。LIPUS应用3周显著增加了多孔HA陶瓷中的矿化组织体积和矿物质含量。伤口愈合试验显示，由于LIPUS治疗，MC 3 T3-E1细胞的迁移增加，部分原因是成骨细胞数量增加。在临床环境中，多孔HA陶瓷与LIPUS联合使用可能是填充大骨缺损的有前景的治疗方法。
Synthetic porous ceramic made of hydroxyapatite (HA) has been used as a bone graft substitute. In the present study we investigated whether low-intensity pulsed ultrasound (LIPUS) accelerates bone ingrowth into the pores of HA ceramic. Application of LIPUS did not mechanically weaken porous ceramic that was immersed in water in vitro. In vivo experiments using rabbits showed that LIPUS application for 2 weeks significantly increased osteoblast number and bone area in the central part of the porous HA ceramic implanted in the femoral condyle in comparison with similarly implanted HA ceramic that was not exposed to LIPUS. LIPUS application for 3 weeks significantly increased mineralized tissue volume and mineral content in the porous HA ceramic. Wound healing assays revealed increased migration of MC3T3-E1 cells as a result of LIPUS treatment, partly accounting for the increased osteoblast number. Use of porous HA ceramic combined with LIPUS may be a promising treatment for filling large bone defects in a clinical setting.